Park Seokmuk, Park Han Woong, Seo Dae Bang, Yoo Dae Sung, Bae Seunghee
Department of Cosmetics Engineering, Konkuk University, Seoul, Republic of Korea.
ASK Company Co., Ltd., Daegu, Republic of Korea.
Front Pharmacol. 2024 Dec 3;15:1482898. doi: 10.3389/fphar.2024.1482898. eCollection 2024.
Scalp hair plays a crucial role in social communication by expressing personal appearance and self-identity. Consequently, hair loss often leads to a perception of unattractiveness, negatively impacting an individual's life and mental health. Currently, the use of Food and Drug Administration (FDA)-approved drugs for hair loss is associated with several side effects, highlighting the need for identifying new drug candidates, such as plant-derived phytochemicals, to overcome these issues.
This study investigated the hair growth-promoting effects of araliadiol, a polyacetylene compound found in plants such as .
We employed an model comprising human hair follicle stem cells (HHFSCs) and human dermal papilla cells (HDPCs) to evaluate the hair growth-promoting effects of araliadiol. The proliferation-stimulating effects of araliadiol were assessed using water-soluble tetrazolium salt assay, adenosine triphosphate content assay, and crystal violet staining assay. In addition, we performed luciferase reporter assay, polymerase chain reaction analysis, cell fractionation, Western blot analysis, and enzyme-linked immunosorbent assay (ELISA) to elucidate the mechanism underlying the hair growth-inductive effects of araliadiol.
Araliadiol exhibited both proliferation- and hair growth-promoting effects in HHFSCs and HDPCs. Specifically, it increased the protein expression of cyclin B1 and Ki67. In HHFSCs, it elevated the expression of hair growth-promoting factors, including CD34, vascular endothelial growth factor (VEGF), and angiopoietin-like 4. Similarly, araliadiol increased the expression of hair growth-inductive proteins such as fibroblast growth factor 7, VEGF, noggin, and insulin-like growth factor 1 in HDPCs. Subsequent Western blot analysis and ELISA using inhibitors such as GW9662 and SB202190 confirmed that these hair growth-promoting effects were dependent on the p38/PPAR-γ signaling in both HHFSCs and HDPCs.
Araliadiol promotes hair growth through the p38/PPAR-γ signaling pathway in human hair follicle cells. Therefore, araliadiol can be considered a novel drug candidate for the treatment of alopecia.
头皮毛发通过展现个人外表和自我身份在社交沟通中发挥着关键作用。因此，脱发常常导致一种缺乏吸引力的认知，对个人生活和心理健康产生负面影响。目前，使用美国食品药品监督管理局（FDA）批准的治疗脱发的药物会带来多种副作用，这凸显了识别新的候选药物（如植物来源的植物化学物质）以克服这些问题的必要性。
本研究调查了刺五加二醇（一种存在于诸如……等植物中的聚乙炔化合物）促进头发生长的作用。
我们采用了一个包含人毛囊干细胞（HHFSCs）和人真皮乳头细胞（HDPCs）的模型来评估刺五加二醇促进头发生长的作用。使用水溶性四氮唑盐测定法、三磷酸腺苷含量测定法和结晶紫染色测定法评估刺五加二醇的增殖刺激作用。此外，我们进行了荧光素酶报告基因测定、聚合酶链反应分析、细胞分级分离、蛋白质印迹分析和酶联免疫吸附测定（ELISA）以阐明刺五加二醇诱导头发生长作用的潜在机制。
刺五加二醇在HHFSCs和HDPCs中均表现出促进增殖和头发生长的作用。具体而言，它增加了细胞周期蛋白B1和Ki67的蛋白质表达。在HHFSCs中，它提高了促进头发生长因子的表达，包括CD34、血管内皮生长因子（VEGF）和血管生成素样4。同样，刺五加二醇增加了HDPCs中诱导头发生长蛋白的表达，如成纤维细胞生长因子7、VEGF、头蛋白和胰岛素样生长因子1。随后使用GW9662和SB202190等抑制剂进行的蛋白质印迹分析和ELISA证实，这些促进头发生长的作用在HHFSCs和HDPCs中均依赖于p38/PPAR-γ信号通路。
刺五加二醇通过人毛囊细胞中的p38/PPAR-γ信号通路促进头发生长。因此，刺五加二醇可被视为治疗脱发的一种新型候选药物。
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