Naqvi Syed Muhammad Zaigham Abbas, Zhao Jingkai, Tahir Muhammad Naveed, Awais Muhammad, Zhang Yanyan, Wu Junfeng, Ahmed Shakeel, Li Linze, Raghavan Vijaya, Hu Jiandong
Department of Electrical Engineering, Henan Agricultural University, Zhengzhou 450002, China.
Henan International Joint Laboratory of Laser Technology in Agriculture Sciences, Zhengzhou 450002, China.
J Agric Food Chem. 2025 Jan 8;73(1):861-872. doi: 10.1021/acs.jafc.4c08229. Epub 2024 Dec 18.
This study explores the development and application of gold and 4-mercaptopyridine (MPY) perovskite-engineered robust nanofibers (GLAMPER-NFs) for the ultrasensitive detection of Abscisic acid (ABA) under Raman spectroscopy, a crucial plant hormone. The GLAMPER-NFs composite material, consisting of MAPbCl nanofibers integrated with MPY-coated gold nanostructures, demonstrates exceptional performance in surface-enhanced Raman scattering (SERS)-based sensing. The study elucidates the material structure and properties through comprehensive characterization using scanning electron microscopy (SEM), UV-vis spectroscopy, fluorescence spectroscopy, Fourier transform infrared, and Raman spectroscopy. The SEM analysis reveals uniform nanofibers with diameter of 107.8 ± 3.06 nm, while spectroscopic studies confirm the successful synthesis and integration of the composite components. The SERS-based detection of ABA showcases remarkable sensitivity, with a linear detection range ( = 0.9957) spanning 7 orders of magnitude (10-10 M) and presented a limit of detection of 10 and enhancement factor of 1.08 × 10. This surpasses the performance of existing sensing platforms, demonstrating clear spectral responses even at femtomolar concentrations. The synergistic effects of the perovskite structure, plasmonic gold nanoparticles, and MPY linking molecules contributed exceptional sensing capabilities to the GLAMPER-NFs material. Fluorescence studies further corroborate the sensitivity and provide insights into the photophysical interactions between ABA and the composite material. This research advances the understanding of perovskite-based hybrid materials and presents a promising GLAMPER-NFs as SERS substrate for ultrasensitive plant hormone detection, with potential applications in agricultural monitoring and plant science research.
本研究探索了金和4-巯基吡啶（MPY）钙钛矿工程化的坚固纳米纤维（GLAMPER-NFs）的开发与应用，用于在拉曼光谱下对脱落酸（ABA）进行超灵敏检测，脱落酸是一种关键的植物激素。GLAMPER-NFs复合材料由集成有MPY包覆金纳米结构的MAPbCl纳米纤维组成，在基于表面增强拉曼散射（SERS）的传感方面表现出卓越性能。该研究通过使用扫描电子显微镜（SEM）、紫外可见光谱、荧光光谱、傅里叶变换红外光谱和拉曼光谱进行全面表征，阐明了材料的结构和性质。SEM分析揭示了直径为107.8±3.06 nm的均匀纳米纤维，而光谱研究证实了复合成分的成功合成与整合。基于SERS的ABA检测显示出显著的灵敏度，线性检测范围（ = 0.9957）跨越7个数量级（10-10 M），检测限为10，增强因子为1.08×10。这超过了现有传感平台的性能，即使在飞摩尔浓度下也呈现出清晰的光谱响应。钙钛矿结构、等离子体金纳米颗粒和MPY连接分子的协同效应赋予了GLAMPER-NFs材料卓越的传感能力。荧光研究进一步证实了灵敏度，并深入了解了ABA与复合材料之间的光物理相互作用。这项研究推进了对基于钙钛矿的混合材料的理解，并展示了一种有前景的GLAMPER-NFs作为用于超灵敏植物激素检测的SERS底物，在农业监测和植物科学研究中具有潜在应用。
