Dube Mbali P, Byaruhanga Charles, Dorny Pierre, Dermauw Veronique, Qekwana Daniel N
Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Pretoria.
Onderstepoort J Vet Res. 2024 Dec 4;91(1):e1-e8. doi: 10.4102/ojvr.v91i1.2157.
Meat inspection is the routine method used to identify cattle infected with Taenia saginata; however, the sensitivity of this method is low. We investigated the prevalence of T. saginata infection in cattle slaughtered in low throughput abattoirs (LTs) in Gauteng province, South Africa, based on meat inspection and serology. A total of 188 cattle carcasses from three abattoirs underwent meat inspection for the presence of T. saginata cysticerci, while serum was tested for the occurrence of antigens using antigen enzyme-linked immunosorbent assay (Ag-ELISA). Polymerase chain reaction (PCR)-sequencing was performed to confirm T. saginata cysts identified during enhanced inspection and incision of 10 randomly selected hearts. Apparent and true prevalence were calculated, and a logistic regression model was fit to evaluate associations between abattoir, sex, animal origin, age and T. saginata serological status. Out of the 188 carcasses, no positive cases (0%) were identified during routine meat inspection, yet three cysticerci-like lesions were identified from three hearts following additional incisions, of which one was confirmed as T. saginata. Fifty-four of the sampled cattle carcasses tested positive using Ag-ELISA (apparent and true prevalence: 29.0%, 71.8%). Feedlot cattle were less likely (odds ratios [OR]: 0.33, p = 0.043) to have a positive serological test result for T. saginata compared to non-feedlot cattle, and the odds of a positive result differed between abattoirs (p 0.05).Contribution: Our results confirmed the low sensitivity of routine meat inspection in LTs, which may pose a public health risk, and therefore other diagnostic methods need to be included in the surveillance system for T. saginata.
肉类检验是用于识别感染牛带绦虫的牛的常规方法；然而，该方法的灵敏度较低。我们基于肉类检验和血清学调查了南非豪登省低产量屠宰场（LTs）屠宰的牛中牛带绦虫感染的流行情况。来自三个屠宰场的总共188头牛的尸体接受了肉类检验，以检查是否存在牛带绦虫囊尾蚴，同时使用抗原酶联免疫吸附测定（Ag-ELISA）检测血清中抗原的出现情况。对随机选择的10个心脏进行增强检查和切开时发现的牛带绦虫囊肿进行聚合酶链反应（PCR）测序以进行确认。计算了表观患病率和真实患病率，并拟合了逻辑回归模型以评估屠宰场、性别、动物来源、年龄与牛带绦虫血清学状态之间的关联。在188具尸体中，常规肉类检验未发现阳性病例（0%），但在额外切开三个心脏后发现了三个类似囊尾蚴的病变，其中一个被确认为牛带绦虫。使用Ag-ELISA对54头采样牛的尸体检测呈阳性（表观和真实患病率：29.0%，71.8%）。与非饲养场的牛相比，饲养场的牛牛带绦虫血清学检测呈阳性的可能性较小（优势比[OR]：0.33，p = 0.043），并且不同屠宰场阳性结果的几率有所不同（p < 0.05）。贡献：我们的结果证实了低产量屠宰场常规肉类检验的低灵敏度，这可能构成公共卫生风险，因此牛带绦虫监测系统需要纳入其他诊断方法。
