ObjectiveThis study was conducted to evaluate the relationship between the frequency of spiritual care and job satisfaction of palliative care nurses.MethodsThis study was a cross-sectional type. The study population consisted of nurses working in palliative care units of three hospitals in eastern Turkey. The study was conducted with 110 nurses who agreed to participate in the study. The researchers' "Information Form", "Nurse Spiritual Care Therapeutics Scale" and "Nurse Job Satisfaction Scale" were used to collect data. Data was collected by face-to-face interview method between August and September 2022.ResultsIt was determined that the nurses scored 45.2 ± 15.8 points from the Nurse Spiritual Care Therapeutics Scale and 4.1 ± 0.7 points from the nurse job satisfaction scale. There was a positive and moderately significant relationship between the frequency of spiritual care giving and job satisfaction of nurses. The frequency of giving spiritual care positively affected job satisfaction (β = .409) and predicted it by 19% (Adjusted R = .195). It was determined that satisfaction with the unit (palliative care) mediated the relationship between the frequency of spiritual care and job satisfaction.Conclusion"Palliative care nurses' frequency of providing spiritual care was moderate and their job satisfaction was high, with frequency of providing spiritual care having a positive effect on job satisfaction. This suggests that increasing opportunities for nurses to provide spiritual care may further enhance their job satisfaction, leading to improved overall well-being.