Yang Chengcong, Zha Musu, Li Lu, Qiao Jiaqi, Kwok Lai-Yu, Wang Dandan, Chen Yongfu
Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010018, China; Key Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010018, China; Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010018, China.
Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010018, China; Key Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010018, China; Inner Mongolia Key Laboratory of Dairy Biotechnology and Engineering, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010018, China.
J Dairy Sci. 2025 Feb;108(2):1211-1226. doi: 10.3168/jds.2024-25614. Epub 2024 Dec 16.
Colorectal cancer (CRC) arises from the accumulation of abnormal mutations in colorectal cells during prolonged inflammation. This study aimed to investigate the potential of probiotic fermented milk containing the probiotic strain, Bifidobacterium animalis ssp. lactis BX-245 (BX-245), in alleviating tumor burden in CRC mice induced by azoxymethane and dextran sodium sulfate. The study monitored changes in tumor size and number, gut microbiota, metabolomics, and inflammation levels before and after the intervention. Our findings indicate that intragastric administration of BX245-fermented milk effectively modulated the intratumor microbiota, as well as the gut microbiota and its metabolism. We also observed a decreased relative abundance of intratumor Akkermansia in the CRC mice, while the intratumor Parabacteroides exhibited a significant positive correlation with tumor number and weight. Moreover, administering BX245-fermented milk significantly reduced gut barrier permeability, alleviated gut barrier damage, and increased serum IL-2 and IFN-γ levels compared with the ordinary fermented milk group. Collectively, our data suggest that administering probiotic fermented milk containing specific functional strains such as BX245 could result in a reduction in tumor burden in CRC mice. Conversely, ordinary fermented milk did not show the same tumor-inhibiting effects. The current results are preliminary, and further confirmation is necessary to establish the causal relationship among probiotic milk, changes in gut microbiota, and disease alleviation.
结直肠癌（CRC）源于长期炎症过程中结肠细胞异常突变的积累。本研究旨在探讨含有益生菌菌株动物双歧杆菌乳亚种乳酸双歧杆菌BX - 245（BX - 245）的益生菌发酵乳在减轻由氧化偶氮甲烷和葡聚糖硫酸钠诱导的CRC小鼠肿瘤负担方面的潜力。该研究监测了干预前后肿瘤大小和数量、肠道微生物群、代谢组学以及炎症水平的变化。我们的研究结果表明，胃内给予BX245发酵乳可有效调节肿瘤内微生物群以及肠道微生物群及其代谢。我们还观察到CRC小鼠肿瘤内阿克曼氏菌的相对丰度降低，而肿瘤内拟杆菌与肿瘤数量和重量呈显著正相关。此外，与普通发酵乳组相比，给予BX245发酵乳显著降低了肠道屏障通透性，减轻了肠道屏障损伤，并提高了血清IL - 2和IFN -γ水平。总体而言，我们的数据表明，给予含有特定功能菌株如BX245的益生菌发酵乳可减轻CRC小鼠的肿瘤负担。相反，普通发酵乳未显示出相同的肿瘤抑制作用。目前的结果是初步的，需要进一步证实以确立益生菌乳、肠道微生物群变化与疾病缓解之间的因果关系。
