Omar Mahmoud A, Mohamed Abobakr A, Alahmadi Yaser, Almaghrabi Mohammed, Bafail Rawan, Mostafa Islam M
Analytical Chemistry Department, Faculty of Pharmacy, Minia University, Minia, Egypt.
Department of Pharmacognosy and Pharmaceutical Chemistry, College of Pharmacy, Taibah University, Al-Madinah Al-Munawwarah, Saudi Arabia.
Luminescence. 2024 Dec;39(12):e70059. doi: 10.1002/bio.70059.
Pizotifen maleate (PZM) is an anti-migraine drug. Effective treatment requires accurate measurement of PZM levels in patients and constant medication concentrations in tablets. This paper presents a new spectrofluorimetric approach for detecting PZM in tablets and human blood plasma (spiked with PZM). The detection mechanism depends on the inhibition of the PZM molecule's photoinduced electron transfer (PET), resulting in a bright fluorescent intensity from PZM. Acetic acid (2.0 M) acidifies the reaction environment and causes PET inhibition. This approach detects PZM at concentrations ranging from 100 to 700 ng mL. Furthermore, the approach has outstanding detection and quantification limits of 30.95 and 93.78 ng mL, respectively. Also, the approach accurately measured PZM content homogeneity in each tablet and spiked human plasma samples without any interference. This new strategy represents a new and sensitive method for accurate PZM monitoring in different samples. Furthermore, the established fluorimetric method's greenness was evaluated.
马来酸哌替芬（PZM）是一种抗偏头痛药物。有效的治疗需要准确测量患者体内的PZM水平以及片剂中恒定的药物浓度。本文提出了一种新的荧光光谱法，用于检测片剂和人血浆（添加了PZM）中的PZM。检测机制取决于对PZM分子光致电子转移（PET）的抑制，从而使PZM产生明亮的荧光强度。乙酸（2.0 M）使反应环境酸化并导致PET抑制。该方法可检测浓度范围为100至700 ng mL的PZM。此外，该方法的检测限和定量限分别出色地达到30.95和93.78 ng mL。而且，该方法准确测量了每片片剂和加标人血浆样品中PZM含量的均匀性，没有任何干扰。这种新策略代表了一种用于在不同样品中准确监测PZM的新的灵敏方法。此外，还评估了所建立的荧光法的绿色度。
