Macêdo Anderson Kelvin Saraiva, da Silva Jicaury Roberta Pereira, Brighenti Ludmila Silva, de Azambuja Ribeiro Rosy Iara, Dos Santos Hélio Batista, Thomé Ralph Gruppi
Laboratório de Processamento de Tecidos - LAPROTEC, Universidade Federal de São João Del-Rei, Campus Centro Oeste, Rua Sebastião Gonçalves Coelho, 400, Divinópolis, Minas Gerais, 35501-296, Brazil.
Universidade do Estado de Minas Gerais, Campus Divinópolis, Avenida Paraná, 3001, Divinópolis, Minas Gerais, 35501- 170, Brazil.
J Mol Histol. 2024 Dec 19;56(1):47. doi: 10.1007/s10735-024-10334-x.
Fish may have different sensitivity to pollutants present in the water. We analyzed the liver histology, and P-gp expression in six species of fish from the Doce River basin. Fish were caught at six different points in the Doce River, and liver samples were taken for histological analysis. P-gp expression was analyzed using an immunohistochemical technique. In Astyanax lacustris, Hoplias intermedius, Hypostomus affinis, Trachelyopterus striatulus and Oligosarcus acutirostris, a double arrangement of hepatocyte plates was generally observed (tubular-form), while in Deutorodon taeniatus, a single arrangement of hepatocyte plates was frequently observed (cord-like). Histological changes, such as cytoplasmic vacuolation and nuclear alteration, were observed in the livers of all species analyzed, however, the species A. lacustris (34.1%) and H. affinis (33.3%) were those with the fewest individuals with histological changes. The H. intermedius, T. striatulus, and O. acutirostris were the species that presented more than 80% of their individuals with histological changes. The A. lacustris and H. affinis were the species that showed the highest P-pg immunolabeling in the liver, while the T. striatulus and O. acutirostris had the lowest levels. These results support the hypothesis that levels of P-gp expression could respond to the resistance or sensitivity of each species to environmental pollutants.
鱼类对水中存在的污染物可能具有不同的敏感性。我们分析了来自多西河盆地的六种鱼类的肝脏组织学和P-糖蛋白表达。在多西河的六个不同地点捕获鱼类，并采集肝脏样本进行组织学分析。使用免疫组织化学技术分析P-糖蛋白表达。在湖栖丽脂鲤、中间霍氏脂鲤、黑口下口鲶、条纹真唇脂鲤和尖吻寡脂鲤中，通常观察到肝细胞板呈双层排列（管状），而在带纹德托罗鱼中，经常观察到肝细胞板呈单层排列（索状）。在所分析的所有物种的肝脏中均观察到组织学变化，如细胞质空泡化和核改变，然而，湖栖丽脂鲤（34.1%）和黑口下口鲶（33.3%）是组织学变化个体最少的物种。中间霍氏脂鲤、条纹真唇脂鲤和尖吻寡脂鲤的个体中，超过80%呈现组织学变化。湖栖丽脂鲤和黑口下口鲶是肝脏中P-pg免疫标记最高的物种，而条纹真唇脂鲤和尖吻寡脂鲤的水平最低。这些结果支持了以下假设，即P-糖蛋白表达水平可能反映每个物种对环境污染物的抗性或敏感性。
