Eren Ozdemir Ayla
Department of health laboratory techniques, Sakarya University, Serdivan Sakarya, Turkey.
BMC Ophthalmol. 2024 Dec 18;24(1):532. doi: 10.1186/s12886-024-03732-y.
We aimed to investigate the effect of diabetic retinopathy and melatonin treatment on the relative telomer lengths (RTL) in retinal pigment epithelium (RPE) cells in a streptozotocin-induced diabetic rat model.
TL can be used to evaluate diabetes mellitus, its complications, and the effectiveness of its treatment. However, TL assessment has not been performed in retinal cells in a diabetic retinopathy model until now.
Forty Sprague-Dawley male rats were randomly divided into four groups. The experimental groups were: Control Group (C): non- diabetic rats; Diabetes Mellitus Group (DM): rats induced to diabetes without treatment; Melatonin and Diabetes Mellitus Group (Mel + DM): rats induced to diabetes and after confirmation, treated with melatonin; Melatonin Group (Mel): rats were not induced to diabetes, treated with melatonin. Diabetes was induced by intraperitoneal administration of streptozotocin solution after 12 h food fasting. For eight weeks after the diabetes was induced, melatonin was administered via subcutaneous injection at a dose of 10 mg / kg. RTLs were measured by qPCR method with modifications. The comparison of averaged data among groups was performed using least significant difference (LSD) and Kruskal - Wallis Test and One way ANOVA test.
RTL was significantly similar in control and melatonin group. RTL was thinnest in DM group, in addition melatonin treatment significantly prevented the RTL shortening in DM + Mel group (p = 0.031).
We demonstrated that diabetic retinopathy led to the shortening of RTL in RPE cells in rats and melatonin treatment prevents this shortening.
我们旨在研究糖尿病视网膜病变和褪黑素治疗对链脲佐菌素诱导的糖尿病大鼠模型中视网膜色素上皮（RPE）细胞相对端粒长度（RTL）的影响。
端粒长度（TL）可用于评估糖尿病及其并发症以及治疗效果。然而，迄今为止尚未在糖尿病视网膜病变模型的视网膜细胞中进行TL评估。
40只Sprague-Dawley雄性大鼠随机分为四组。实验组为：对照组（C）：非糖尿病大鼠；糖尿病组（DM）：诱导为糖尿病但未治疗的大鼠；褪黑素与糖尿病组（Mel + DM）：诱导为糖尿病并确认后用褪黑素治疗的大鼠；褪黑素组（Mel）：未诱导为糖尿病但用褪黑素治疗的大鼠。禁食12小时后腹腔注射链脲佐菌素溶液诱导糖尿病。糖尿病诱导后八周，通过皮下注射以10mg/kg的剂量给予褪黑素。采用改良的qPCR方法测量RTL。使用最小显著差异（LSD）、Kruskal-Wallis检验和单因素方差分析对组间平均数据进行比较。
对照组和褪黑素组的RTL显著相似。DM组的RTL最薄，此外褪黑素治疗显著防止了DM + Mel组的RTL缩短（p = 0.031）。
我们证明糖尿病视网膜病变导致大鼠RPE细胞中RTL缩短，而褪黑素治疗可防止这种缩短。
