使用拉丝试验评估用于检测高毒力肺炎克雷伯菌的最佳琼脂培养基。
Evaluation of an optimal agar medium for detecting hypervirulent Klebsiella pneumoniae using string test.
作者信息
Watanabe Naoki, Masuda Akari, Watari Tomohisa, Otsuka Yoshihito, Yamagata Kazufumi, Fujioka Miyuki
机构信息
Clinical Laboratory, Kameda Medical Center, Higashi-cho 929, Kamogawa, Chiba 296-8602, Japan.
Graduate School of Health Sciences, Hirosaki University, Hon-cho 66-1, Hirosaki, Aomori, 036-8564, Japan.
出版信息
Access Microbiol. 2024 Sep 20;6(9). doi: 10.1099/acmi.0.000834.v3. eCollection 2024.
The string test is a screening method for detecting hypervirulent (hvKp). Agar media are used for string tests; however, the effect of the type of media on the test results remains unclear. We aimed to determine the optimal agar medium and cutoff value for the string test. We performed the string test on 99 strains using different agar media: sheep blood, chocolate, Drigalski's and MacConkey. The diagnostic accuracy was calculated in concordance with the , or gene levels. The diagnostic accuracy rates for sheep blood, chocolate, Drigalski's and MacConkey agar were 0.79, 0.75, 0.73 and 0.64, respectively. When the cutoff was changed from 5 to 10 mm, the diagnostic accuracy rate for sheep blood agar decreased from 0.79 to 0.65. Our findings suggest that the type of agar medium impacts the string test results and sheep blood agar with a 5-mm cutoff is the optimal condition for detecting hvKp.
拉丝试验是一种检测高毒力肺炎克雷伯菌（hvKp）的筛查方法。琼脂培养基用于拉丝试验；然而，培养基类型对试验结果的影响仍不明确。我们旨在确定拉丝试验的最佳琼脂培养基和临界值。我们使用不同的琼脂培养基（羊血琼脂、巧克力琼脂、德里加尔斯基琼脂和麦康凯琼脂）对99株菌株进行了拉丝试验。根据 、 或 基因水平计算诊断准确性。羊血琼脂、巧克力琼脂、德里加尔斯基琼脂和麦康凯琼脂的诊断准确率分别为0.79、0.75、0.73和0.64。当临界值从5毫米变为10毫米时，羊血琼脂的诊断准确率从0.79降至0.65。我们的研究结果表明，琼脂培养基的类型会影响拉丝试验结果，临界值为5毫米的羊血琼脂是检测hvKp的最佳条件。
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