• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

骨髓间充质干细胞来源的外泌体通过miR-144-3p/SLC7A11轴抑制肝星状细胞的激活。

Bone marrow mesenchymal stem cell-originated exosomes suppress activation of hepatic stellate cells through the miR-144-3p/SLC7A11 axis.

作者信息

Hao Yanqin, Wang Rong, Zhou Qing, Ren Jiaolong

机构信息

Department of Infectious Diseases, First Hospital of Shanxi Medical University, Taiyuan, Shanxi, P.R. China.

The First Clinical Medical School, Shanxi Medical University, Taiyuan, Shanxi, P.R. China.

出版信息

Clin Exp Hepatol. 2024 Sep;10(3):197-210. doi: 10.5114/ceh.2024.142898. Epub 2024 Sep 30.

DOI:10.5114/ceh.2024.142898
PMID:39697374
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11650813/
Abstract

AIM OF THE STUDY

This study aimed to investigate the impact of bone marrow-derived mesenchymal stem cell exosomes (BMSC-Exos) on hepatic stellate cell (HSC) activation and explore the underlying molecular mechanisms in liver fibrosis.

MATERIAL AND METHODS

BMSC-Exos were co-incubated with LPS-activated LX-2 cells. Fibrosis markers, iron content, malondialdehyde (MDA), glutathione (GSH), reactive oxygen species (ROS) levels, and ferroptosis-related proteins were assessed. The role of miR-144-3p originating from BMSC-Exos in LX-2 cell activation was studied through dual-luciferase reporter gene and RNA pull-down experiments.

RESULTS

Treatment with BMSC-Exos up-regulated miR-144-3p in LX-2 cells, down-regulated SLC7A11, increased iron content and ROS levels, and reduced fibrosis markers and GSH. BMSC-Exos mediated ferroptosis and inhibited HSC activation by transmitting miR-144-3p targeting SLC7A11.

CONCLUSIONS

BMSC-Exos regulate SLC7A11 expression through miR-144-3p transfer, promoting ferroptosis and suppressing HSC activation in liver fibrosis.

摘要

研究目的

本研究旨在探讨骨髓间充质干细胞外泌体（BMSC-Exos）对肝星状细胞（HSC）激活的影响，并探索肝纤维化潜在的分子机制。

材料与方法

将BMSC-Exos与脂多糖激活的LX-2细胞共同孵育。评估纤维化标志物、铁含量、丙二醛（MDA）、谷胱甘肽（GSH）、活性氧（ROS）水平以及铁死亡相关蛋白。通过双荧光素酶报告基因和RNA下拉实验研究源自BMSC-Exos的miR-144-3p在LX-2细胞激活中的作用。

结果

用BMSC-Exos处理上调了LX-2细胞中miR-144-3p的表达，下调了溶质载体家族7成员11（SLC7A11），增加了铁含量和ROS水平，并降低了纤维化标志物和GSH。BMSC-Exos通过传递靶向SLC7A11的miR-144-3p介导铁死亡并抑制HSC激活。

结论

BMSC-Exos通过miR-144-3p转移调节SLC7A11表达，促进铁死亡并抑制肝纤维化中的HSC激活。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61e6/11650813/9fd4c61b491b/CEH-10-54744-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61e6/11650813/236964e54391/CEH-10-54744-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61e6/11650813/967287250929/CEH-10-54744-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61e6/11650813/e3521d685d45/CEH-10-54744-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61e6/11650813/65ac3d87d0e7/CEH-10-54744-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61e6/11650813/9fd4c61b491b/CEH-10-54744-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61e6/11650813/236964e54391/CEH-10-54744-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61e6/11650813/967287250929/CEH-10-54744-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61e6/11650813/e3521d685d45/CEH-10-54744-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61e6/11650813/65ac3d87d0e7/CEH-10-54744-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/61e6/11650813/9fd4c61b491b/CEH-10-54744-g005.jpg

相似文献

1
Bone marrow mesenchymal stem cell-originated exosomes suppress activation of hepatic stellate cells through the miR-144-3p/SLC7A11 axis.骨髓间充质干细胞来源的外泌体通过miR-144-3p/SLC7A11轴抑制肝星状细胞的激活。
Clin Exp Hepatol. 2024 Sep;10(3):197-210. doi: 10.5114/ceh.2024.142898. Epub 2024 Sep 30.
2
Mesenchymal stem cell-derived exosomal microRNA-367-3p alleviates experimental autoimmune encephalomyelitis via inhibition of microglial ferroptosis by targeting EZH2.间质干细胞来源的外泌体 microRNA-367-3p 通过靶向 EZH2 抑制小胶质细胞铁死亡缓解实验性自身免疫性脑脊髓炎。
Biomed Pharmacother. 2023 Jun;162:114593. doi: 10.1016/j.biopha.2023.114593. Epub 2023 Mar 29.
3
BMSC-derived Exosomes Ameliorate Peritoneal Dialysis-associated Peritoneal Fibrosis via the Mir-27a-3p/TP53 Pathway.骨髓间充质干细胞来源的外泌体通过 miR-27a-3p/TP53 通路改善腹膜透析相关性腹膜纤维化。
Curr Med Sci. 2024 Apr;44(2):333-345. doi: 10.1007/s11596-024-2853-7. Epub 2024 Apr 16.
4
Bone marrow mesenchymal stem cell-derived exosomes protect podocytes from HBx-induced ferroptosis.骨髓间充质干细胞来源的外泌体保护足细胞免受 HBx 诱导的铁死亡。
PeerJ. 2023 May 11;11:e15314. doi: 10.7717/peerj.15314. eCollection 2023.
5
Chondroprotective effects of bone marrow mesenchymal stem cell-derived exosomes in osteoarthritis.骨髓间充质干细胞来源的外泌体对骨关节炎的软骨保护作用。
J Bioenerg Biomembr. 2024 Feb;56(1):31-44. doi: 10.1007/s10863-023-09991-6. Epub 2023 Nov 28.
6
Exosomes derived from bone marrow mesenchymal stem cells regulate pyroptosis via the miR-143-3p/myeloid differentiation factor 88 axis to ameliorate intestinal ischemia-reperfusion injury.骨髓间充质干细胞来源的外泌体通过 miR-143-3p/髓样分化因子 88 轴调节细胞焦亡，改善肠缺血再灌注损伤。
Bioengineered. 2023 Dec;14(1):2253414. doi: 10.1080/21655979.2023.2253414.
7
Exosomes Derived from BMSCs Ameliorate Intestinal Ischemia-Reperfusion Injury by Regulating miR-144-3p-Mediated Oxidative Stress.骨髓间充质干细胞来源的外泌体通过调控miR-144-3p介导的氧化应激改善肠道缺血再灌注损伤
Dig Dis Sci. 2022 Nov;67(11):5090-5106. doi: 10.1007/s10620-022-07546-0. Epub 2022 May 27.
8
BMSC-exosomes miR-25-3p Regulates the p53 Signaling Pathway Through PTEN to Inhibit Cell Apoptosis and Ameliorate Liver Ischemia‒reperfusion Injury.骨髓间充质干细胞外泌体 miR-25-3p 通过 PTEN 调控 p53 信号通路抑制细胞凋亡并减轻肝缺血再灌注损伤。
Stem Cell Rev Rep. 2023 Nov;19(8):2820-2836. doi: 10.1007/s12015-023-10599-x. Epub 2023 Aug 18.
9
Exosomes from osteoarthritic fibroblast-like synoviocytes promote cartilage ferroptosis and damage via delivering microRNA-19b-3p to target SLC7A11 in osteoarthritis.骨关节炎成纤维样滑膜细胞来源的外泌体通过将 microRNA-19b-3p 递送至 SLC7A11 靶点促进软骨铁死亡和损伤在骨关节炎中。
Front Immunol. 2023 Aug 24;14:1181156. doi: 10.3389/fimmu.2023.1181156. eCollection 2023.
10
Mesenchymal stem cell-derived exosomal miR-26a induces ferroptosis, suppresses hepatic stellate cell activation, and ameliorates liver fibrosis by modulating SLC7A11.间充质干细胞衍生的外泌体miR-26a通过调节溶质载体家族7成员11（SLC7A11）诱导铁死亡，抑制肝星状细胞活化，并改善肝纤维化。
Open Med (Wars). 2024 May 13;19(1):20240945. doi: 10.1515/med-2024-0945. eCollection 2024.

本文引用的文献

1
Human umbilical cord mesenchymal stem cells inhibit liver fibrosis via the microRNA-148a-5p/SLIT3 axis.人脐带间充质干细胞通过 microRNA-148a-5p/SLIT3 轴抑制肝纤维化。
Int Immunopharmacol. 2023 Dec;125(Pt A):111134. doi: 10.1016/j.intimp.2023.111134. Epub 2023 Oct 31.
2
Ulinastatin ameliorates podocyte ferroptosis via regulating miR-144-3p/SLC7A11 axis in acute kidney injury.乌司他丁通过调控 miR-144-3p/SLC7A11 轴减轻急性肾损伤足细胞铁死亡。
In Vitro Cell Dev Biol Anim. 2023 Oct;59(9):697-705. doi: 10.1007/s11626-023-00814-x. Epub 2023 Oct 11.
3
Programmed Cell Death in Liver Fibrosis.
肝纤维化中的程序性细胞死亡
J Inflamm Res. 2023 Sep 1;16:3897-3910. doi: 10.2147/JIR.S427868. eCollection 2023.
4
Exosome-mediated miR-144-3p promotes ferroptosis to inhibit osteosarcoma proliferation, migration, and invasion through regulating ZEB1.外泌体介导的 miR-144-3p 通过调节 ZEB1 促进铁死亡抑制骨肉瘤增殖、迁移和侵袭。
Mol Cancer. 2023 Jul 17;22(1):113. doi: 10.1186/s12943-023-01804-z.
5
Novel ginsenoside monomer RT4 promotes colitis repair in mice by regulating miR-144-3p/SLC7A11 signaling pathway.新型人参皂苷单体 RT4 通过调控 miR-144-3p/SLC7A11 信号通路促进小鼠结肠炎修复。
Fundam Clin Pharmacol. 2023 Dec;37(6):1129-1138. doi: 10.1111/fcp.12934. Epub 2023 Jun 23.
6
Metformin ameliorates ferroptosis in cardiac ischemia and reperfusion by reducing NOX4 expression via promoting AMPKα.二甲双胍通过促进 AMPKα的表达降低 NOX4 表达从而减轻心脏缺血再灌注中的铁死亡。
Pharm Biol. 2023 Dec;61(1):886-896. doi: 10.1080/13880209.2023.2212700.
7
SLC7A11-associated ferroptosis in acute injury diseases: mechanisms and strategies.SLC7A11 相关的铁死亡在急性损伤疾病中的作用机制和策略。
Eur Rev Med Pharmacol Sci. 2023 May;27(10):4386-4398. doi: 10.26355/eurrev_202305_32444.
8
Exosomal miR-192-5p secreted by bone marrow mesenchymal stem cells inhibits hepatic stellate cell activation and targets PPP2R3A.骨髓间充质干细胞分泌的外泌体 miR-192-5p 抑制肝星状细胞活化并靶向 PPP2R3A。
J Histotechnol. 2023 Dec;46(4):158-169. doi: 10.1080/01478885.2023.2215151. Epub 2023 May 25.
9
Stellate cell in hepatic inflammation and acute injury.肝炎症和急性损伤中的星状细胞。
J Cell Physiol. 2023 Jun;238(6):1226-1236. doi: 10.1002/jcp.31029. Epub 2023 Apr 30.
10
Ginsenoside Re attenuates myocardial ischemia/reperfusion induced ferroptosis via miR-144-3p/SLC7A11.人参皂苷 Re 通过 miR-144-3p/SLC7A11 减轻心肌缺血/再灌注诱导的铁死亡。
Phytomedicine. 2023 May;113:154681. doi: 10.1016/j.phymed.2023.154681. Epub 2023 Jan 30.