In Vitro Approaches for Detection of Protein-Lipid Interactions and Protein-to-Protein Transport of Lipids Using Bifunctional Lipid Analogs.

Functionalized lipid analogs are a versatile tool for studying lipid localization, metabolism and interactions in cells. As a common follow-up research, in vitro experiments are utilized to confirm specific interactors and characterize protein-lipid interactions. Here, we describe protocols that rely on commercially available photoactivatable and clickable lipids in reconstituted systems to capture protein-lipid interactions and detect lipid transport between purified proteins.