Fullem Kiersten R, Obradovi Aleksa, MacLellan Michelle P, Poudel Mousami, Minsavage Gerald V, Goss Erica M, Potnis Neha, Jones Jeffrey B, Paret Mathews L
Department of Plant Pathology, University of Florida, Gainesville, FL, USA.
Plant Pathology Department, Faculty of Agriculture, University of Belgrade, Belgrade, Serbia.
Int J Syst Evol Microbiol. 2024 Dec;74(12). doi: 10.1099/ijsem.0.006613.
Six strains isolated from muskmelon and watermelon seedlings affected by stem rot and wilting in Serbia were reported as based on pathogenicity, LOPAT and cell wall fatty acid analyses. Recent bacterial isolates from cucurbit crops displaying -like symptoms in Alabama, USA, were identified as , prompting polyphasic re-evaluation of the Serbian strains. All six strains were found to cause severe disease in watermelon and squash seedlings under greenhouse conditions. Strains KFB 138 and KFB 140 underwent whole-genome sequencing and were found to have the highest level of 16S rRNA similarity to LJ2 (both 99.87%). Phylogenies based on housekeeping genes and core-genome analysis placed both strains into phylogroup 11 of the species complex, with KFB 138 forming a lineage basal to all other phylogroup 11 members. In core-genome phylogeny, KFB 140 was placed into a clade alongside LJ2. Average nucleotide identity based on blast (ANIb) identified KFB 140 as a member of (95.85%), though KFB 138 did not produce an ANIb value over 95% to any type strain to which it was compared. Values for DNA-DNA hybridization for both strains were below 70% to all reference strains tested, though KFB 140 was found to be most similar to (68.2%). KFB 138 and KFB 140 were further characterized using the online Type Genome Server, biochemical profiling with the Biolog Gen III MicroPlate system, matrix-assisted laser desorption/ionization time of flight mass spectrometry and imaged with transmission electron microscopy. From the results of the above analyses, we conclude that KFB 140 is a member of the species and that KFB 138 represents a novel species, for which we propose the name (KFB 138, NCPPB 4762=LMG 33366), named for its location of isolation.
据报道，从塞尔维亚受茎腐病和枯萎病影响的甜瓜和西瓜幼苗中分离出的6个菌株，是基于致病性、LOPAT和细胞壁脂肪酸分析得出的。最近在美国阿拉巴马州从表现出类似症状的葫芦科作物中分离出的细菌菌株被鉴定为，这促使对塞尔维亚菌株进行多相重新评估。在温室条件下，所有6个菌株均被发现可导致西瓜和南瓜幼苗严重发病。对菌株KFB 138和KFB 140进行了全基因组测序，发现它们与LJ2的16S rRNA相似度最高（均为99.87%）。基于管家基因和核心基因组分析的系统发育将这两个菌株置于物种复合体的系统发育群11中，KFB 138形成了一个位于所有其他系统发育群11成员基部的谱系。在核心基因组系统发育中，KFB 140与LJ2一起被置于一个进化枝中。基于blast的平均核苷酸同一性（ANIb）将KFB 140鉴定为的一个成员（95.85%），尽管KFB 138与它所比较的任何类型菌株的ANIb值均未超过95%。与所有测试参考菌株的DNA-DNA杂交值均低于70%，尽管发现KFB 140与最相似（68.2%）。使用在线型基因组服务器对KFB 138和KFB 140进行了进一步表征，使用Biolog Gen III微孔板系统进行生化分析，基质辅助激光解吸/电离飞行时间质谱分析，并通过透射电子显微镜成像。根据上述分析结果，我们得出结论，KFB 140是的一个成员，而KFB 138代表一个新的物种，我们将其命名为（KFB 138，NCPPB 4762 = LMG 33366），以其分离地点命名。
