一种改进的聚（V）硫代寡核苷酸合成平台。
An Improved P(V) Thio-Oligonucleotide Synthesis Platform.
作者信息
Nassir Molhm, Gherardi Luca, Redman Richard L, Jin Yonghao, Yao Feng, Yang Yang, Raheja Nicholas, Natarajan Arjun, Butler David, Knouse Kyle W, Baran Phil S
机构信息
Department of Chemistry, Scripps Research, 10550 North Torrey Pines Road, La Jolla, California 92037, United States.
Dipartimento di Chimica e Chimica Industriale, University of Pisa, Via G. Moruzzi 13, Pisa 56124, Italy.
出版信息
Org Lett. 2025 Jan 10;27(1):97-102. doi: 10.1021/acs.orglett.4c03980. Epub 2024 Dec 19.
Abstract
Three critical advances in simplifying the adoption of P(V)-based stereopure, phosphorothioate-containing oligonucleotide synthesis are reported. A more inexpensive phosphorus-sulfur incorporation reagent () is introduced, a robust linker system was developed, and a systematic study of common nucleobase protecting groups was performed to significantly reduce the barrier to adoption of this technology.
摘要
本文报道了在简化基于磷（V）的立体纯含硫代磷酸酯寡核苷酸合成方法方面取得的三项关键进展。引入了一种更廉价的磷硫掺入试剂（），开发了一种稳健的连接系统，并对常见的核苷酸碱基保护基团进行了系统研究，以显著降低采用该技术的障碍。
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