Liu Fei, Zeng Jiaxiong, Ding Jijuan, Wang Cheng, He Zhili, Liu Zhiwei, Shu Longfei
School of Life Sciences, Jiaying University, Meizhou 514015, China; Conservation and Utilization Laboratory of Mountain Characteristic Resources in Guangdong Province, Meizhou 514015, China; School of Environmental Science and Engineering, Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology, State Key Laboratory for Biocontrol, Sun Yat-sen University, Guangzhou 510275, China.
School of Environmental Science and Engineering, Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology, State Key Laboratory for Biocontrol, Sun Yat-sen University, Guangzhou 510275, China.
Sci Total Environ. 2025 Jan 1;958:178118. doi: 10.1016/j.scitotenv.2024.178118. Epub 2024 Dec 18.
The phosphorus (P) cycle plays a crucial role in the biogeochemical cycling of mangrove sediments. However, the diversity of microbially-driven P-cycling and its coupling with nitrogen (N)-cycling remain poorly understood. In this study, we used metagenomic approaches to investigate microbial P-cycling and its potential interactions with N-cycling in mangrove sediments. Our results revealed that pH, total carbon, and total nitrogen were key environmental factors influencing the diversity of P-cycling microbial communities. Phosphorus metabolic pathways differed among mangrove sediment depths. In surface sediments (0-5 cm), microbial communities primarily acquired readily available inorganic phosphorus, whereas, in deeper sediments (>60 cm), they hydrolyzed more persistent triphosphates, reabsorbed nucleotides, and sourced free phosphate, reflecting a shift in phosphorus transport modes. We also identified glutamate metabolism as a potential pathway linking P-cycling with N-cycling, with these functions co-occurring in both contigs and genomes. Additionally, the diversity of microbial communities associated with the P-cycling increased with sediment depth, suggesting that microbially-driven P-cycling diversifies as depth increases. This study provides new insights into P-cycling and its potential coupling with N-cycling through glutamate metabolism, its coupling with N-cycling through glutamate metabolism.
磷（P）循环在红树林沉积物的生物地球化学循环中起着关键作用。然而，微生物驱动的磷循环的多样性及其与氮（N）循环的耦合仍知之甚少。在本研究中，我们使用宏基因组学方法研究红树林沉积物中微生物的磷循环及其与氮循环的潜在相互作用。我们的结果表明，pH值、总碳和总氮是影响磷循环微生物群落多样性的关键环境因素。不同深度的红树林沉积物中磷代谢途径存在差异。在表层沉积物（0-5厘米）中，微生物群落主要获取易利用的无机磷，而在较深的沉积物（>60厘米）中，它们水解更持久的三磷酸盐，重新吸收核苷酸并获取游离磷酸盐，这反映了磷运输模式的转变。我们还确定谷氨酸代谢是将磷循环与氮循环联系起来的潜在途径，这些功能在重叠群和基因组中同时出现。此外，与磷循环相关的微生物群落的多样性随沉积物深度增加而增加，这表明微生物驱动的磷循环随深度增加而多样化。本研究通过谷氨酸代谢为磷循环及其与氮循环的潜在耦合提供了新的见解，即通过谷氨酸代谢与氮循环耦合。
