Gnanagurusamy Jayapradha, Krishnamoorthy Sneha, Muruganatham Bharathi, Selvamurugan Nagarajan, Muthusami Sridhar
Department of Biochemistry, Karpagam Academy of Higher Education, Coimbatore 641021, Tamil Nadu, India; Centre for Cancer Research, Karpagam Academy of Higher Education, Coimbatore 641021, Tamil Nadu, India.
Department of Biochemistry, Karpagam Academy of Higher Education, Coimbatore 641021, Tamil Nadu, India; Centre for Bioinformatics, Karpagam Academy of Higher Education, Coimbatore 641021, Tamil Nadu, India.
Gene. 2025 Feb 20;938:149166. doi: 10.1016/j.gene.2024.149166. Epub 2024 Dec 18.
The role of transforming growth factor-beta (TGF-β) is dual, such that, it inhibits tumor development in initial stage and promotes metastasis in later stage. The present study is aimed to analyse the relevance of different types of TGF-β and their receptors on the overall survival (OS) and TGF-β driven gene expression in individuals with cervical cancer (CC) using ONCODB and GEPIA databases. The in-silico gene expression analysis showed, TGF-β1 and TGFβR2 are upregulated in cells infected with human papilloma virus (HPV)16, whereas, TGF-β2, TGFβR1 and TGFβR3 expression were downregulated. In HPV 18 infected cells, TGF-β1, TGF-β2 and TGFβR1 were downregulated, meanwhile, TGF-β3, TGFβR2 and TGFβR3 were upregulated. OS analysis of CC patients with different TGF-β expression revealed that, TGF-β1, TGF-β2, TGF-β3 and TGFβR2 were associated with reduced survival rate. Further, we identified four microRNAs (miRNAs) (hsa-miR-21-5p, hsa-miR-29b-3p, hsa-miR-101-3p and hsa-miR-130a-3p) interacted favorably with TGF-β in HPV 16 and 18 positive samples using MIENTURNET. This present review further emphasizes that, targeting TGF-β could be a novel and futuristic approach for CC management and therapeutics.
转化生长因子-β（TGF-β）的作用具有双重性，即在肿瘤发展初期它会抑制肿瘤，而在后期则促进转移。本研究旨在利用ONCODB和GEPIA数据库分析不同类型的TGF-β及其受体与宫颈癌（CC）患者总生存期（OS）以及TGF-β驱动的基因表达之间的相关性。计算机基因表达分析显示，在感染人乳头瘤病毒（HPV）16的细胞中，TGF-β1和TGFβR2上调，而TGF-β2、TGFβR1和TGFβR3表达下调。在感染HPV 18的细胞中，TGF-β1、TGF-β2和TGFβR1下调，同时，TGF-β3、TGFβR2和TGFβR3上调。对不同TGF-β表达的CC患者进行的OS分析显示，TGF-β1、TGF-β2、TGF-β3和TGFβR2与生存率降低相关。此外，我们使用MIENTURNET在HPV 16和18阳性样本中鉴定出四种微小RNA（miRNA）（hsa-miR-21-5p、hsa-miR-29b-3p、hsa-miR-101-3p和hsa-miR-130a-3p）与TGF-β存在良好的相互作用。本综述进一步强调，靶向TGF-β可能是一种用于CC管理和治疗的新颖且具有未来前景的方法。
