Kuo Catherine K, Petersen Bryan C, Tuan Rocky S
Cartilage Biology and Orthopaedics Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Department of Health and Human Services, Bethesda, Maryland 20892-8022, USA.
Dev Dyn. 2008 May;237(5):1477-89. doi: 10.1002/dvdy.21547.
Tendon is one of the least understood tissues of the musculoskeletal system in terms of development and morphogenesis. Collagen fibrillogenesis has been the most studied aspect of tendon development, focusing largely on the role of matrix molecules such as collagen type III and decorin. While involvement of matrix molecules in collagen fibrillogenesis during chick tendon development is well understood, the role of growth factors has yet to be elucidated. This work examines the expression patterns of transforming growth factor (TGF) -beta1, -beta2, and -beta3, and their receptors with respect to expression patterns of collagen type III, decorin, and fibronectin. We focus on the intermediate stages of tendon development in the chick embryo, a period during which the tendon micro- and macro-architecture are being established. Our findings demonstrate for the first time that TGF-beta1, -beta2, and -beta3 have distinct spatiotemporal developmental protein localization patterns in the developing tendon and strongly suggest that these isoforms have independent roles in tendon development.
就发育和形态发生而言，肌腱是肌肉骨骼系统中最不为人所了解的组织之一。胶原纤维形成一直是肌腱发育研究最多的方面，主要集中在诸如III型胶原和核心蛋白聚糖等基质分子的作用上。虽然基质分子在鸡肌腱发育过程中参与胶原纤维形成已得到充分了解，但生长因子的作用尚未阐明。这项工作研究了转化生长因子（TGF）-β1、-β2和-β3及其受体相对于III型胶原、核心蛋白聚糖和纤连蛋白的表达模式。我们关注鸡胚胎中肌腱发育的中间阶段，在此期间肌腱的微观和宏观结构正在形成。我们的研究结果首次证明，TGF-β1、-β2和-β3在发育中的肌腱中具有独特的时空发育蛋白定位模式，并强烈表明这些异构体在肌腱发育中具有独立作用。
