Haas Allison L, Bradley Benjamin T, Hanson Kimberly E
Department of Pathology, University of Utah, Salt Lake City, UT, USA; ARUP Laboratories, 500 Chipeta Way, Salt Lake City, UT 84108, USA.
Department of Pathology, University of Utah, Salt Lake City, UT, USA; ARUP Laboratories, 500 Chipeta Way, Salt Lake City, UT 84108, USA; Division of Infectious Diseases, Department of Medicine, University of Utah, Salt Lake City, UT, USA; Mycology Section, ARUP Laboratories, Salt Lake City, Utah, USA.
Infect Dis Clin North Am. 2025 Mar;39(1):41-56. doi: 10.1016/j.idc.2024.11.004. Epub 2024 Dec 18.
Culture-independent diagnostics for fungi potentially offer increased sensitivity and more rapid results relative to culture. Recent developments include new platforms for fungal cell wall antigen detection, commercially available targeted nucleic acid amplification tests, and the use of sequencing-based technologies for the detection and identification of a broad range of fungal pathogens. Although these tests are not without limitations, many more commercially available platforms now exist, and efforts to increase the standardization of laboratory-developed tests are ongoing. Additionally, implementation studies are beginning to delineate the optimal use cases for molecular diagnostics across a range of fungal diseases and at-risk populations.
与培养法相比，不依赖培养的真菌诊断方法可能具有更高的灵敏度和更快的检测结果。最近的进展包括用于真菌细胞壁抗原检测的新平台、市售的靶向核酸扩增检测，以及使用基于测序的技术来检测和鉴定多种真菌病原体。尽管这些检测方法并非没有局限性，但现在有更多市售平台，并且正在努力提高实验室自行开发检测方法的标准化程度。此外，实施研究开始明确分子诊断在一系列真菌疾病和高危人群中的最佳应用案例。
