Johnson M D
Am J Physiol. 1985 Feb;248(2 Pt 2):F314-8. doi: 10.1152/ajprenal.1985.248.2.F314.
Extracts of mammalian atrial tissue contain potent natriuretic substances known collectively as atrial natriuretic factor (ANF). The purposes of the present experiments were: 1) to improve on existing bioassay methodology for the detection of ANF activity in atrial extracts, and 2) to compare the ANF activity of atrial extracts prepared from Brattleboro-stain diabetes insipidus (DI) rats with that from normal and water-deprived Long-Evans (LE) rats. A pool of atrial tissue extract (AE) was prepared from normal Sprague-Dawley rats for use as a standard against which unknown AE samples could be compared. Five doses, ranging from 27 to 432 micrograms of AE protein, were assayed in the Sprague-Dawley bioassay rats. Phosphate-buffered saline (PBS) vehicle and ventricular tissue extracts were also assayed. Statistical analysis of several log dose-response relationships revealed that the bioassay response most appropriate in determining relative natriuretic activity of AE was the log of the experimental/control ratio for sodium excretion. The bioassay was used to demonstrate that PBS atrial extracts from both water-deprived LE rats and DI rats contain more natriuretic activity than do PBS atrial extracts from LE rats.
哺乳动物心房组织提取物中含有一类统称为心房利钠因子（ANF）的强效利钠物质。本实验的目的是：1）改进现有的生物测定方法，以检测心房提取物中的ANF活性；2）比较从Brattleboro品系尿崩症（DI）大鼠制备的心房提取物与正常及缺水的Long-Evans（LE）大鼠心房提取物的ANF活性。从正常的Sprague-Dawley大鼠制备了一批心房组织提取物（AE），用作标准品，可与未知的AE样品进行比较。在Sprague-Dawley生物测定大鼠中测定了5种剂量（27至432微克AE蛋白）。还测定了磷酸盐缓冲盐水（PBS）载体和心室组织提取物。对几种对数剂量-反应关系的统计分析表明，在确定AE相对利钠活性时最适用的生物测定反应是钠排泄的实验/对照比值的对数。该生物测定法用于证明，缺水的LE大鼠和DI大鼠的PBS心房提取物比LE大鼠的PBS心房提取物含有更多的利钠活性。
