Alosaimi Manal E, Almalki Atiah H, Abduljabbar Maram H, Alnemari Reem M, Alaqel Saleh I, Serag Ahmed
Department of Basic Sciences, College of Medicine, Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia.
Department of Pharmaceutical Chemistry, College of Pharmacy, Taif University, Taif, Saudi Arabia.
Luminescence. 2024 Dec;39(12):e70060. doi: 10.1002/bio.70060.
In this study, the use of functionalized graphene quantum dots (GQDs) as a fluorescent probe has been investigated for the quantitative determination of galantamine, a choline esterase inhibitor used for the treatment of Alzheimer's disease. The GQDs exhibit a significant quenching in their fluorescence intensity upon interaction with galantamine allowing for sensitive and selective detection of the drug. This quenching process follows a dynamic pattern with a linear relationship between fluorescence intensity and the concentration of galantamine. Several factors affecting the quenching process were investigated and optimized, including the concentration of GQDs, the pH of the solution, and the incubation time. The proposed probe exhibited excellent analytical performance with a linear range of 10-500 ng/mL, a limit of detection of 15 ng/mL, accuracy of 100.78 ± 0.698%, and intraday and interday precision of 0.742 and 1.369%, respectively. Furthermore, the GQDs-based sensor exhibited good selectivity towards galantamine in the presence of potentially interfering substances. Another advantage of the GQDs-based sensor is its greenness evaluation, as it offers a more environmentally friendly alternative compared to traditional methods. In addition, the GQDs-based sensor was successfully applied to analyze galantamine in pharmaceutical samples and in vivo samples, demonstrating its potential for pharmacokinetics monitoring.
在本研究中，已对功能化石墨烯量子点（GQDs）作为荧光探针用于定量测定加兰他敏进行了研究。加兰他敏是一种用于治疗阿尔茨海默病的胆碱酯酶抑制剂。GQDs与加兰他敏相互作用时荧光强度显著猝灭，从而实现对该药物的灵敏且选择性检测。这种猝灭过程遵循动态模式，荧光强度与加兰他敏浓度之间呈线性关系。研究并优化了影响猝灭过程的几个因素，包括GQDs的浓度、溶液的pH值和孵育时间。所提出的探针表现出优异的分析性能，线性范围为10 - 500 ng/mL，检测限为15 ng/mL，准确度为100.78 ± 0.698%，日内和日间精密度分别为0.742%和1.369%。此外，基于GQDs的传感器在存在潜在干扰物质的情况下对加兰他敏表现出良好的选择性。基于GQDs的传感器的另一个优点是其绿色度评估，因为与传统方法相比，它提供了一种更环保的选择。此外，基于GQDs的传感器已成功应用于分析药物样品和体内样品中的加兰他敏，证明了其在药代动力学监测方面的潜力。
