Azimi Seyedeh Afrooz, Sadegh Nia Hamid Reza, Bahrami Naghmeh, Dargahi Hossein, Jamaati Hamidreza, Pasdar Alireza, Kazempour Dizaji Mehdi, Shirian Sadegh, Zerehsaz Bahareh, Mohamadnia Abdolreza
Department of Modern Sciences & Technologies, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
Division of Neurocognitive Sciences, Psychiatry and Behavioral Sciences Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
Tanaffos. 2024 Jan;23(1):58-64.
Cancer is a disease caused by manifestation and abnormal gene expression. Many of the genes that inhibit cancer by the microRNAs. The aim of this study was to investigate the Expression of miR155 gene and CEA and VEGF proteins as Diagnostic Markers in Early Stages of Non-Small Cell Lung Cancer (NSCLC).
Fifty pairs of non-small lung cancer specimens of patients from healthy and tumors were collected based on physical examination and diagnosis of an expert, that, in Masih Daneshvari Hospital. 50 healthy volunteers as a control group were volunteered by the physician after examination and filled out the consent form in this study. From all subjects, 6 ml of peripheral blood were taken and examined by Real-Time PCR (RT-PCR) and ELISA.
The expression level of miR-155 in patients was significantly increased compared to the control group (p<0.001). VEGF protein was positive in 34 of the 50 patients and in healthy subjects, 3 persons were positive. The statistical comparison of the amount of positive biomarker was performed in two groups and it was shown that there is a statistically significant difference between these two groups (P<0.001). CEA protein was positive in 38 of the 50 patients and 5 in healthy subjects were positive. The statistical comparison of the amount of positive biomarker was performed in two groups and it was shown that there is a statistically significant difference between these two groups (P<0.001).
This study showed that miR155 gene and CEA and VEGF proteins are relatively good markers for the diagnosis of non-small cell lung cancer patients in Iranian population.
癌症是一种由基因表达异常和显现所引发的疾病。许多基因通过微小RNA抑制癌症。本研究的目的是探究miR155基因、癌胚抗原（CEA）和血管内皮生长因子（VEGF）蛋白作为非小细胞肺癌（NSCLC）早期诊断标志物的表达情况。
基于马西赫·达内什瓦里医院专家的体格检查和诊断，收集了50对来自健康者和肿瘤患者的非小肺癌标本。50名健康志愿者作为对照组，由医生在检查后招募，并在本研究中填写了知情同意书。从所有受试者中采集6毫升外周血，通过实时定量聚合酶链反应（RT-PCR）和酶联免疫吸附测定（ELISA）进行检测。
与对照组相比，患者中miR-155的表达水平显著升高（p<0.001）。50名患者中有34名VEGF蛋白呈阳性，健康受试者中有3人呈阳性。对两组中阳性生物标志物的数量进行统计学比较，结果显示两组之间存在统计学显著差异（P<0.001）。50名患者中有38名CEA蛋白呈阳性，健康受试者中有5人呈阳性。对两组中阳性生物标志物的数量进行统计学比较，结果显示两组之间存在统计学显著差异（P<0.001）。
本研究表明，miR155基因、CEA和VEGF蛋白是伊朗人群中非小细胞肺癌患者诊断的相对良好标志物。
