调节神经干/祖细胞的增殖和分化。
regulates the proliferation and differentiation of neural stem/progenitor cells.
作者信息
Feng Xuanran, Du Xue, Yang Xiaoyu, Chen Changqi, Liang Zhanping, Xu Xiaonan, Wang Yi, Zheng Jialin C, Xia Xiaohuan, Liu Jianhui
机构信息
Department of Anesthesiology, Tongji Hospital, School of Medicine, Tongji University, Shanghai, China.
Translational Research Center, Shanghai Yangzhi Rehabilitation Hospital, School of Medicine, Tongji University, Shanghai, China.
出版信息
Front Cell Dev Biol. 2024 Dec 5;12:1510746. doi: 10.3389/fcell.2024.1510746. eCollection 2024.
BACKGROUND
MicroRNAs (miRNAs) have emerged as an essential regulator of the cell fate commitment of neural stem/progenitor cells (NPCs), although the impacts of certain miRNAs on NPCs remain vague. The aim of this study is to investigate the regulatory effects of on the cell fate commitment of NPCs.
METHODS
We investigated the impact of on the proliferation and differentiation capacities of primary NPCs by manipulating the expression of using specific mimics and inhibitors. The effects of on NPCs was confirmed through stereotactic injection of antagonists to the brains of mice at postnatal day 1 (P1).
RESULTS
The expression levels of kept increasing in the differentiation process of NPCs and . Perturbation of 's function showed that inhibited NPCs' proliferation and promoted embryonic NPCs to differentiate more favorably to the glial lineage. We then validated the anti-proliferation and pro-glial roles of using NPCs isolated from P1 mouse brains. study further showed enlarged NPCs pools and inhibited gliogenesis in the brains of P1 mice after animals received antagomir-185-5p.
CONCLUSION
Our study suggests as an important regulator for the proliferation and glial fate commitment of NPCs.
背景
微小RNA（miRNA）已成为神经干/祖细胞（NPC）细胞命运决定的重要调节因子，尽管某些miRNA对NPC的影响仍不明确。本研究旨在探讨[具体miRNA名称]对NPC细胞命运决定的调控作用。
方法
我们通过使用特异性模拟物和抑制剂操纵[具体miRNA名称]的表达，研究其对原代NPC增殖和分化能力的影响。通过在出生后第1天（P1）将[具体miRNA名称]拮抗剂立体定向注射到小鼠脑内，证实了[具体miRNA名称]对NPC的作用。
结果
在NPC向[具体细胞类型1]和[具体细胞类型2]分化的过程中，[具体miRNA名称]的表达水平持续升高。对[具体miRNA名称]功能的干扰表明，[具体miRNA名称]抑制NPC的增殖，并促进胚胎NPC更倾向于向神经胶质谱系分化。然后，我们使用从P1小鼠脑中分离的NPC验证了[具体miRNA名称]的抗增殖和促神经胶质生成作用。进一步的研究表明，在动物接受抗miR-185-5p后，P1小鼠脑内NPC池扩大，神经胶质生成受到抑制。
结论
我们的研究表明，[具体miRNA名称]是NPC增殖和神经胶质命运决定的重要调节因子。
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