Corval Amanda Rocha da Costa, de Carvalho Lucas Amoroso Lopes, Mesquita Emily, Fiorotti Jéssica, Corrêa Thaís Almeida, Bório Victória Silvestre, Carneiro Adriani da Silva, Pinheiro Daniel Guariz, Coelho Irene da Silva, Santos Huarrisson Azevedo, Fernandes Everton Kort Kamp, Angelo Isabele da Costa, Bittencourt Vânia R E P, Golo Patrícia Silva
Postgraduate Program in Veterinary Sciences, Veterinary Institute, Federal Rural University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
Laboratory of Bioinformatics, Department of Agricultural, Livestock and Environmental Biotechnology, School of Agricultural and Veterinary Sciences, São Paulo State University (UNESP), Jaboticabal, SP, Brazil.
Front Microbiol. 2024 Dec 5;15:1507931. doi: 10.3389/fmicb.2024.1507931. eCollection 2024.
is widely known for its role as an arthropod biocontrol agent and plant bioinoculant. By using mass-production industrial methods, it is possible to produce large amounts of fungal single-celled propagules (including blastospores) to be applied in the field. However, in the environment, the solar ultraviolet components (particularly UV-B) can harm the fungus, negatively impacting its pathogenicity toward the arthropod pest. The present study is the first to use comparative genome-wide transcriptome analyses to unveil changes in gene expression between blastospores exposed or not to UV-B. Relative blastospores culturability was calculated 72 h after UV-B exposure and exhibited 100% culturability. In total, 6.57% ( = 728) out of 11,076 predicted genes in were differentially expressed after UV-B exposure: 320 genes (44%; 320/728) were upregulated and 408 (56%; 408/720) were downregulated in the UV-B exposed blastospores. Results unveiled differentially expressed gene sets related to fungal virulence, production of secondary metabolites, and DNA repair associated with UV damage; genes related to virulence factors were downregulated, and genes associated with nucleotide excision repair were upregulated. These findings illustrate critical aspects of blastospores strategies to overcome UV-B damage and survive solar radiation exposures in insulated fields.
它作为节肢动物生物防治剂和植物生物接种剂而广为人知。通过使用大规模生产的工业方法，可以生产大量的真菌单细胞繁殖体（包括芽生孢子）用于田间应用。然而，在环境中，太阳紫外线成分（特别是UV-B）会损害真菌，对其对节肢动物害虫的致病性产生负面影响。本研究首次使用全基因组转录组比较分析来揭示暴露于UV-B和未暴露于UV-B的芽生孢子之间的基因表达变化。在UV-B照射72小时后计算相对芽生孢子可培养性，其可培养性为100%。在 中预测的11,076个基因中，共有6.57%（=728个）在UV-B照射后差异表达：在暴露于UV-B的芽生孢子中，320个基因（44%；320/728）上调，408个基因（56%；408/720）下调。结果揭示了与真菌毒力、次生代谢产物产生以及与紫外线损伤相关的DNA修复相关的差异表达基因集；与毒力因子相关的基因下调，与核苷酸切除修复相关的基因上调。这些发现阐明了芽生孢子在隔离田间克服UV-B损伤并在太阳辐射暴露下存活的策略的关键方面。
