Wu Yanru, Yang Wenquan, Kou Jiancun, Li Qinyao, Liu Jiaqing, Chi Lu, Zhang Yangcan, Liu Qian, Yu Yanghua
College of Grassland Agriculture, Northwest A&F University, Yangling, China.
Key Laboratory of the Alpine Grassland Ecology in the Three Rivers Region (Qinghai University), Ministry of Education, Xining, China.
Front Microbiol. 2024 Dec 5;15:1500070. doi: 10.3389/fmicb.2024.1500070. eCollection 2024.
Due to the cold climate and low soil nutrient content, high-altitude mining areas are challenging to restore ecologically. Their poor nutrient content may be ameliorated by introducing specific microorganisms into the soil. This study aims to evaluate the effects of a highly efficient phosphate solubilizing bacterium MWP-1, , on plant growth, soil nutrients in remedying the soil of the high-altitude Muli mining area in Qinghai Province, and analyze its impact on microbial communities through high-throughput sequencing soil microbial communities. The results showed that MWP-1 significantly increased the content of soil available phosphorus by >50%, soil organic matter and total nitrogen by >10%, and significantly increased the height, coverage, and aboveground biomass of vegetation by >40% in comparison with the control ( < 0.05). MWP-1 mainly affected the composition of the soil bacterial communities at the taxonomic level below the phylum. Its impact on soil fungal communities occurred at the phylum and below taxonomic levels. In addition, MWP-1 also significantly improved the diversity of soil bacterial and fungal communities ( 0.05), and changed their functions. It also significantly altered the relative abundance of genes regulating phosphorus absorption and transport, inorganic phosphorus dissolution and organic phosphorus mineralization in the bacterial community ( 0.05). It caused a significant increase in the relative abundance of the genes regulating nitrogen fixation and nitrification in nitrogen cycling ( 0.05), but a significant decrease in the genes regulating phospholipase ( 0.05). Although sequencing results indicated that did not become the dominant species, its dissolved phosphorus elements can promote plant growth and development, enrich soil nutrient content, and affect the succession of microbial communities, enhance ecosystem stability, with an overall positive effect on soil remediation in the mining area.
由于气候寒冷且土壤养分含量低，高海拔矿区的生态恢复具有挑战性。通过向土壤中引入特定微生物，可改善其贫瘠的养分含量。本研究旨在评估高效解磷细菌MWP-1对青海省高海拔木里矿区土壤修复中植物生长、土壤养分的影响，并通过高通量测序土壤微生物群落分析其对微生物群落的影响。结果表明，与对照相比，MWP-1显著提高了土壤有效磷含量>50%，土壤有机质和全氮含量>10%，并显著提高了植被高度、覆盖度和地上生物量>40%（<0.05）。MWP-1主要在门以下的分类水平上影响土壤细菌群落的组成。其对土壤真菌群落的影响发生在门及以下分类水平。此外，MWP-1还显著提高了土壤细菌和真菌群落的多样性（0.05），并改变了它们的功能。它还显著改变了细菌群落中调节磷吸收和运输、无机磷溶解和有机磷矿化的基因相对丰度（0.05）。它导致氮循环中调节固氮和硝化作用的基因相对丰度显著增加（0.05），但调节磷脂酶的基因显著减少（0.05）。虽然测序结果表明MWP-1没有成为优势种，但其溶解的磷元素可促进植物生长发育，丰富土壤养分含量，影响微生物群落演替，增强生态系统稳定性，对矿区土壤修复具有总体积极作用。
