Dental hygienists in China face unique occupational challenges, with their job satisfaction being pivotal to performance. This study investigates the interplay between job satisfaction and occupational stress, burnout, and musculoskeletal disorders (MSDs) among this group. A cross-sectional survey was conducted involving 21 female dental hygienists from Sir Run Run Shaw Hospital. Data were collected on general information, occupational stress (using the Perceived Stress Scale), burnout (Maslach Burnout Inventory), MSDs, and job satisfaction. High levels of occupational stress and burnout were reported, yet these did not significantly correlate with job satisfaction (P > .05). In contrast, MSDs showed a significant negative correlation with job satisfaction (P = .004). Factors significantly associated with job satisfaction included effective collaboration, quality of work, institutional support, patient care, and work flexibility. While stress and burnout are prevalent among Chinese dental hygienists, they do not directly impact job satisfaction. MSDs, however, significantly affect satisfaction levels. The study suggests that addressing MSDs and enhancing workplace factors can improve job satisfaction and retention in the profession.