Miao Shan, Sun Junyong, Li Ying, Zhang Qiang, Chen Hongqi, Gao Feng
Laboratory of Functionalized Molecular Solids, Ministry of Education, Anhui Key Laboratory of Chemo/Biosensing, Laboratory of Optical Probes and Bioelectrocatalysis (LOPAB), Anhui Province Key Laboratory of Biomedical Materials and Chemical Measurement, College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241002, PR China.
Laboratory of Functionalized Molecular Solids, Ministry of Education, Anhui Key Laboratory of Chemo/Biosensing, Laboratory of Optical Probes and Bioelectrocatalysis (LOPAB), Anhui Province Key Laboratory of Biomedical Materials and Chemical Measurement, College of Chemistry and Materials Science, Anhui Normal University, Wuhu 241002, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2025 Mar 15;329:125598. doi: 10.1016/j.saa.2024.125598. Epub 2024 Dec 17.
Developing near-infrared fluorescent probes for simultaneous tracking of lipid droplets (LDs) and lysosomes is highly desirable for studying cell metabolism. In this work, deep-red/near-infrared dual-emission carbonized polymer dots (DN-CPDs) were prepared for ratiometric monitoring of the intracellular polarity. Detailed structural analysis revealed that the deep-red emission and near-infrared peak of DN-CPDs originate from the molecular state and surface state, respectively. The surface-state emission was derived from the intraparticle charge-transfer (ICT) effect of the donor-bridge-acceptor (D-π-A) structure of DN-CPDs. The obtained DN-CPDs exhibited excellent dual-labeling ability, large Stokes shifts, ratiometric polarity sensitivity, high selectivity, and satisfactory photostability. Moreover, with the polarity distinction between LDs and lysosomes, DN-CPDs nanoprobes were successfully used to observe the dynamic changes of the two aforementioned organelles during starvation-induced lipophagy and drug-induced lipophagy inhibition processes. This work not only provides a useful tool for LD-lysosome related studies but is also valuable for the preparation of CPDs with long wavelength emission.
开发用于同时追踪脂滴（LDs）和溶酶体的近红外荧光探针对于研究细胞代谢非常有必要。在这项工作中，制备了深红色/近红外双发射碳化聚合物点（DN-CPDs）用于细胞内极性的比率监测。详细的结构分析表明，DN-CPDs的深红色发射和近红外峰分别源于分子态和表面态。表面态发射源自DN-CPDs供体-桥-受体（D-π-A）结构的颗粒内电荷转移（ICT）效应。所获得的DN-CPDs表现出优异的双标记能力、大斯托克斯位移、比率极性敏感性、高选择性和令人满意的光稳定性。此外，由于LDs和溶酶体之间的极性差异，DN-CPDs纳米探针成功用于观察饥饿诱导的自噬性溶酶体降解和药物诱导的自噬性溶酶体降解抑制过程中上述两种细胞器的动态变化。这项工作不仅为LD-溶酶体相关研究提供了有用的工具，对于制备具有长波长发射的CPDs也具有重要价值。
