干血斑：一种使用单滴血液检测乙肝病毒、丙肝病毒和艾滋病毒核酸的诊断检测方法。

Dried blood spot: A diagnostic detection method for HBV, HCV and HIV nucleic acid using a single drop of blood.

作者信息

Nilsson Stephen Strunge, Demant Jonas, Thønnings Sara, Weis Nina, Westh Henrik, Pinholt Mette

机构信息

Department of Clinical Microbiology, Copenhagen University Hospital, Hvidovre, Denmark; Department of Clinical Microbiology, Copenhagen University Hospital, Herlev, Denmark.

Detpartment of Infectious Diseases, Copenhagen University Hospital, Hvidovre, Denmark.

出版信息

Diagn Microbiol Infect Dis. 2025 Mar;111(3):116661. doi: 10.1016/j.diagmicrobio.2024.116661. Epub 2024 Dec 17.

DOI:10.1016/j.diagmicrobio.2024.116661

PMID:39706101

Abstract

The global strategy to eradicate Hepatitis B (HBV), Hepatitis C (HCV), and HIV by 2030 is critical due to their impact and challenges to healthcare systems. HCV is curable, but HBV and HIV are only suppressible, with a vaccine available solely for HBV. Innovative diagnostic methods are needed, especially for high-risk populations like people who inject drugs (PWID). This study validates a dried blood spot (DBS) nucleic acid amplification test (NAAT) using the Hologic Panther system for detecting HBV, HCV, and HIV. The method was used to screen among PWID in the Capital Region of Denmark. The DBS method demonstrated high sensitivity, with a 95 % limit of detection (LoD) of 2711 IU/mL for HBV, 525 IU/mL for HCV, and 4022 copies/mL for HIV. Screening of 83 PWID in Denmark revealed a 13 % prevalence of active HCV infection, offering significant benefits in settings where traditional venous access is difficult.

摘要

到2030年消除乙型肝炎（HBV）、丙型肝炎（HCV）和艾滋病毒的全球战略至关重要，因为它们对医疗系统有影响并带来挑战。丙型肝炎是可治愈的，但乙型肝炎和艾滋病毒只能得到抑制，且只有乙型肝炎有疫苗可用。需要创新的诊断方法，特别是针对注射吸毒者等高危人群。本研究验证了一种使用Hologic Panther系统的干血斑（DBS）核酸扩增检测（NAAT）方法，用于检测HBV、HCV和艾滋病毒。该方法用于丹麦首都地区的注射吸毒者筛查。DBS方法显示出高灵敏度，HBV的95%检测限（LoD）为2711 IU/mL，HCV为525 IU/mL，艾滋病毒为4022拷贝/mL。对丹麦83名注射吸毒者的筛查显示，活动性丙型肝炎感染患病率为13%，这在传统静脉通路困难的环境中具有显著益处。