Xie Yuchen, Feng Yihui, Li Shaohua, Yu Bowen, Yang Fangzheng, Li Yanfei, Cheng Yuanchao, Yu Zhouxi, Li Chanjuan, Dong Jing, Yuan Junhua
Department of Special Medicine, School of Basic Medicine, Qingdao University, Qingdao 266000, China.
School of Rehabilitation Sciences and Engineering, University of Health and Rehabilitation Sciences, Qingdao 266000, China.
Peptides. 2025 Jan;183:171336. doi: 10.1016/j.peptides.2024.171336. Epub 2024 Dec 18.
Obesity is a worldwide health issue. Effective and safe methods for obesity management are highly desirable. In the current study, hyperbaric oxygen (HBO) treatment was investigated as a potential treatment against obesity-associated hyperphagia and hyperenergy intake. Diet induced obesity (DIO) mice model was established with high fat diet (HFD) feeding, HBO was then co-administered. Food and energy intake were assessed with nocturnal food intake assay. Immunohistochemistry for c-Fos was performed for neuronal activation in arcuate nucleus (ARC), paraventricular nucleus of hypothalamus (PVN) and lateral parabrachial nucleus (LPBN) of brain. Additionally, enzyme-linked immunosorbent assay (ELISA) in serum and immunofluorescence in LPBN were performed. Results indicated that HBO co-treatment effectively decreased food and energy intake in DIO mice, reverted the abnormal neuronal activation in the ARC and PVN, and enhanced both peripheral and central nesfatin-1 peptide levels without affecting serum leptin levels. While SHU9119 microinjection in LPBN effectively abolished the beneficial effects of HBO on body weight, visceral fat, nocturnal feeding and energy intake in DIO mice. In conclusion, HBO treatment could effectively protect against HFD-induced increase of food and energy intake, which is associated with its central effects against abnormal neuronal activation in ARC and PVN and enhanced peptide levels of nesfatin-1 both centrally and peripherally. The melanocortin system downstream of nesfatin-1 may exert a potential effect in this process.
肥胖是一个全球性的健康问题。非常需要有效且安全的肥胖管理方法。在当前的研究中，对高压氧（HBO）治疗作为一种针对肥胖相关的食欲亢进和能量摄入过多的潜在治疗方法进行了研究。通过高脂饮食（HFD）喂养建立饮食诱导肥胖（DIO）小鼠模型，然后联合给予HBO。通过夜间食物摄入试验评估食物和能量摄入。对大脑的弓状核（ARC）、下丘脑室旁核（PVN）和外侧臂旁核（LPBN）进行c-Fos免疫组织化学检测以评估神经元激活情况。此外，还进行了血清酶联免疫吸附测定（ELISA）和LPBN的免疫荧光检测。结果表明，联合HBO治疗可有效降低DIO小鼠的食物和能量摄入，逆转ARC和PVN中异常的神经元激活，并提高外周和中枢nesfatin-1肽水平，而不影响血清瘦素水平。而在LPBN中微量注射SHU9119可有效消除HBO对DIO小鼠体重、内脏脂肪、夜间进食和能量摄入的有益作用。总之，HBO治疗可有效防止HFD诱导的食物和能量摄入增加，这与其对ARC和PVN中异常神经元激活的中枢作用以及中枢和外周nesfatin-1肽水平升高有关。nesfatin-1下游的黑皮质素系统可能在此过程中发挥潜在作用。
