Yu Chenliang, Jiang Zhengchu, Xie Qiandan, Wang Qi, Wang Lei, Guan Yuanyuan, Li Yi, Yu Weiwu, Dai Wensheng, Zhang Rui, Wu Jiasheng
State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Hangzhou, People's Republic of China; School of Forestry and Biotechnology, Zhejiang A&F University, Hangzhou, People's Republic of China.
Department of Landscape Architecture, Jiyang College, Zhejiang A&F University, Hangzhou, People's Republic of China.
Int J Biol Macromol. 2025 Feb;291:138944. doi: 10.1016/j.ijbiomac.2024.138944. Epub 2024 Dec 18.
Foliar fertilizers quickly replenish nutrients for plant growth, boosting production and quality. However, how this affects metabolite accumulation in fruits is unclear. In this study, the metabolome and transcriptome of Torreya grandis seeds were investigated after five different foliar fertilizer treatments. Based on the results, foliar fertilizer treatments significantly altered the visual properties and nutritional quality of T. grandis seeds. According to the transcriptome and metabolome data, the differential metabolites and genes in T. grandis seeds were enriched in the sugar/starch and lipid metabolism-related pathways. Correlation analysis revealed that TgVIN1 and TgPEPCK play key roles in sugar/starch and lipid metabolism pathways, respectively. A dual-luciferase analysis and yeast one-hybrid assay were used to examine the regulation of candidate transcription factors on TgVIN1 and TgPEPCK expression. The results showed that TgHDZIP1 and TgMYB7 could directly bind to the TgVIN1 promoter and activate TgVIN1 expression. Similarly, TgIWS1 could directly bind to the TgPEPCK promoter. Transient overexpression of TgVIN1 increased the contents of fructose, soluble sugar and starch in and TgPEPCK significantly increased the C16:1 content in tobacco leaves, respectively. Our results contribute to the mechanisms underlying sucrose/starch and lipid metabolism as affected by foliar fertilizer treatments.
叶面肥能迅速为植物生长补充养分，提高产量和品质。然而，其对果实中代谢物积累的影响尚不清楚。在本研究中，对五种不同叶面肥处理后的香榧种子进行了代谢组和转录组分析。结果表明，叶面肥处理显著改变了香榧种子的外观特性和营养品质。根据转录组和代谢组数据，香榧种子中的差异代谢物和基因富集在糖/淀粉和脂质代谢相关途径中。相关性分析表明，TgVIN1和TgPEPCK分别在糖/淀粉和脂质代谢途径中起关键作用。采用双荧光素酶分析和酵母单杂交试验检测候选转录因子对TgVIN1和TgPEPCK表达的调控。结果表明，TgHDZIP1和TgMYB7可直接结合TgVIN1启动子并激活TgVIN1表达。同样，TgIWS1可直接结合TgPEPCK启动子。瞬时过表达TgVIN1分别增加了烟草叶片中果糖、可溶性糖和淀粉的含量，而过表达TgPEPCK显著增加了烟草叶片中C16:1的含量。我们的研究结果有助于揭示叶面肥处理对蔗糖/淀粉和脂质代谢的影响机制。
