Wang Ruideng, He Xi, Su Shilong, Bai Jinwu, Liu Haifeng, Zhou Fang
Department of Orthopedics, Peking University Third Hospital, Beijing, China; Engineering Research Center of Bone and Joint Precision Medicine, Beijing, China.
Key Laboratory of Biomechanics and Mechanobiology (Beihang University), Ministry of Education; Key Laboratory of Innovation and Transformation of Advanced Medical Devices, Ministry of Industry and Information Technology; National Medical Innovation Platform for Industry-Education Integration in Advanced Medical Devices (Interdiscipline of Medicine and Engineering); School of Biological Science and Medical Engineering, Beihang University, Beijing, 100191, China.
Int J Biol Macromol. 2025 Mar;294:138945. doi: 10.1016/j.ijbiomac.2024.138945. Epub 2024 Dec 18.
The repair of diabetic bone defects is still filled with enormous challenges. Excessive reactive oxygen species (ROS) are regenerated in diabetic bone defect sites which is harmful to bone regeneration. Therefore, it's to a good strategy to scavenge the excess ROS to provide a friendly environment for diabetic bone defects repair. Herein, a novel composite hydrogel with ROS-scavenging and osteogenic ability is constructed. This methacrylated silk fibroin based composite hydrogel is capable of releasing tannin acid and inorganic ion, which can reduce oxidative stress, restore homeostasis and enhance osteogenesis. In vitro results indicated that the composite hydrogel could promote osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) under oxidative stress condition. Furthermore, in vivo results suggested that it can significantly promote bone regeneration in diabetic bone defects. In conclusion, this study provides critical insight into the biological mechanism and potential therapy for diabetic bone regeneration.
糖尿病性骨缺损的修复仍然面临着巨大挑战。糖尿病性骨缺损部位会产生过量的活性氧（ROS），这对骨再生有害。因此，清除过量的ROS为糖尿病性骨缺损修复提供有利环境是一个不错的策略。在此，构建了一种具有ROS清除能力和成骨能力的新型复合水凝胶。这种基于甲基丙烯酸化丝素蛋白的复合水凝胶能够释放单宁酸和无机离子，可减轻氧化应激、恢复内环境稳态并增强成骨作用。体外实验结果表明，该复合水凝胶在氧化应激条件下可促进骨髓间充质干细胞（BMSC）的成骨分化。此外，体内实验结果表明它能显著促进糖尿病性骨缺损的骨再生。总之，本研究为糖尿病性骨再生的生物学机制及潜在治疗方法提供了重要见解。
