Xiaojie Xing, Jinbing Chen, Yiling Cheng, JingJing Su, Yuan Liu, Yu Pan, Hao Yu, Hui Cheng
Fujian Key Laboratory of Oral Diseases & Fujian Provincial Engineering Research Center of Oral Biomaterial & Stomatological Key Lab of Fujian College and University, School and Hospital of Stomatology, Fujian Medical University, 88 Jiaotong Road, Fuzhou, Fujian 350004, China.
Stomatological Hospital of Xiamen Medical College, Xiamen, Fujian 361008, China.
Int J Biol Macromol. 2025 Mar;296:139712. doi: 10.1016/j.ijbiomac.2025.139712. Epub 2025 Jan 9.
Alveolar bone defects caused by inflammation or trauma jeopardize patients' oral functions. Guided bone regeneration (GBR) is widely used in repairing periodontal tissue, with barrier membranes play a crucial role in preserving the bone regeneration space. In this study, an injectable dual-crosslinked hydrogel was developed to improve the existing barrier membranes in flexibility and functionality. The hydrogel matrix, composed of methacrylated carboxymethyl chitosan (CMCS) reinforced with robust silk fibroin (SF), was further functionalized with bioactive glass (BG) particles to promote bone regeneration. The pre-gel solution achieved a fast-curing process under visible light and at body temperature. Further, the composite hydrogels presented good biocompatibility, biodegradability, resilience, alongside in vitro barrier effect against human gingival fibroblasts (HGFs). It significantly enhanced osteogenic differentiation and angiogenesis of bone marrow mesenchymal stem cells (BMSCs), facilitate the tube formation of human umbilical vein endothelial cells (HUVECs), and inhibit Staphylococcus aureus and Porphyromonas gingivalis. In a rat skull defect model, the osteogenic performance of hydrogels was comparable with that of collagen membranes (Bio-Gide®). Overall, this in-situ gel-forming barrier material served as a stable carrier for bioactive ions and a biomineralized scaffold for tissue ingrowth, supporting the enhancement of GBR technique.
由炎症或创伤引起的牙槽骨缺损会危及患者的口腔功能。引导骨再生（GBR）被广泛用于修复牙周组织，其中屏障膜在保留骨再生空间方面起着关键作用。在本研究中，开发了一种可注射的双交联水凝胶，以改善现有屏障膜的柔韧性和功能性。水凝胶基质由用坚固的丝素蛋白（SF）增强的甲基丙烯酸化羧甲基壳聚糖（CMCS）组成，并用生物活性玻璃（BG）颗粒进一步功能化以促进骨再生。预凝胶溶液在可见光和体温下实现了快速固化过程。此外，复合水凝胶具有良好的生物相容性、生物降解性、弹性，以及对人牙龈成纤维细胞（HGFs）的体外屏障作用。它显著增强了骨髓间充质干细胞（BMSCs）的成骨分化和血管生成，促进了人脐静脉内皮细胞（HUVECs）的管形成，并抑制了金黄色葡萄球菌和牙龈卟啉单胞菌。在大鼠颅骨缺损模型中，水凝胶的成骨性能与胶原膜（Bio-Gide®）相当。总体而言，这种原位凝胶形成的屏障材料可作为生物活性离子的稳定载体和组织向内生长的生物矿化支架，支持GBR技术的改进。
