Jonjaroen Veasarach, Jitrakorn Sarocha, Charoonnart Patai, Kaewsaengon Parichart, Thinkohkaew Korlid, Payongsri Panwajee, Surarit Rudee, Saksmerprome Vanvimon, Niamsiri Nuttawee
Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.
Center of Excellence for Shrimp Molecular Biology and Biotechnology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand; National Center of Genetic Engineering and Biotechnology, (BIOTEC), Thailand Science Park, Pathum Thani 12120, Thailand.
Int J Biol Macromol. 2025 Feb;290:138970. doi: 10.1016/j.ijbiomac.2024.138970. Epub 2024 Dec 18.
Delivering double-stranded RNA (dsRNA) in shrimp is challenging due to the lack of an effective carrier system. This study optimized chitosan nanoparticles (CNs) from two sources-α-chitosan from shrimp and β-chitosan from squid-to encapsulate antiviral dsRNA for oral administration via shrimp feed. Using response surface methodology (RSM), formulations were refined for encapsulation efficiency, particle size, polydispersity index, and zeta potential. Both types of CNs demonstrated high encapsulation efficiency (>95 %), small sizes (<300 nm), and stable zeta potential (>20 mV). Shrimp-derived CNs provided superior RNase protection and controlled release, while squid-derived CNs showed a burst release. Incorporated into feed, both types of CNs remained stable for over a month. Shrimp-derived CNs offered greater dsRNA protection (>70 %) and improved efficacy against white spot syndrome virus (WSSV), significantly reducing mortality. These results position shrimp-derived CNs as promising dsRNA carriers for combating WSSV in shrimp aquaculture.
由于缺乏有效的载体系统，在对虾中递送双链RNA（dsRNA）具有挑战性。本研究优化了来自两种来源的壳聚糖纳米颗粒（CNs）——虾源α-壳聚糖和鱿鱼源β-壳聚糖——以通过对虾饲料口服递送抗病毒dsRNA。使用响应面法（RSM），对制剂的包封效率、粒径、多分散指数和zeta电位进行了优化。两种类型的CNs均表现出高包封效率（>95%）、小尺寸（<300nm）和稳定的zeta电位（>20mV）。虾源CNs提供了更好的核糖核酸酶保护和控释，而鱿鱼源CNs则表现出突释。掺入饲料后，两种类型的CNs在一个多月内保持稳定。虾源CNs提供了更好的dsRNA保护（>70%），并提高了抗白斑综合征病毒（WSSV）的效果，显著降低了死亡率。这些结果表明，虾源CNs有望成为对虾养殖中对抗WSSV的dsRNA载体。
