Frackowiak D, Lorrain L, Wrobel D, Leblanc R M
Biochem Biophys Res Commun. 1985 Jan 16;126(1):254-61. doi: 10.1016/0006-291x(85)90599-6.
The polarized photoacoustic, absorption and fluorescence spectra of chloroplasts and thylakoids in unstretched and stretched polyvinyl alcohol films were measured. The intensity ratios of fluorescence bands at 674 nm, 700 nm, 730 nm and 750 nm, and the polarized fluorescence excitation spectra are strongly dependent on light polarization and film stretching. In stretched films, thylakoids exhibit predominantly 674 nm emission. The ratio of photoacoustic signal to absorption is different for light polarized parallel and perpendicular to film stretching. This difference is large in the region of chlorophyll a and carotenoids absorption in which the fluorescence excitation spectra are also strongly dependent on light polarization and film stretching. The observed spectral changes are explained by reorientation of pigment molecules influencing the yield of excitation transfer between different pigments.
测量了未拉伸和拉伸的聚乙烯醇薄膜中叶绿体和类囊体的偏振光声、吸收和荧光光谱。674nm、700nm、730nm和750nm处荧光带的强度比以及偏振荧光激发光谱强烈依赖于光偏振和薄膜拉伸。在拉伸薄膜中，类囊体主要发射674nm的光。平行和垂直于薄膜拉伸方向偏振的光，其光声信号与吸收的比值不同。在叶绿素a和类胡萝卜素吸收区域，这种差异很大，其中荧光激发光谱也强烈依赖于光偏振和薄膜拉伸。观察到的光谱变化是由色素分子的重新取向来解释的，这种重新取向影响了不同色素之间激发转移的产率。
