Du Jiajun, Xue Junyu, Tian Xutong, Luo Juyue, Ömür Ali Doğan, Yang Jianying, Li Yumeng
College of Basic Medicine and Forensic Medicine, Henan University of Science and Technology, Luoyang, Henan, China.
College of Clinical Medicine, The First Affiliated Hospital, Henan University of Science and Technology, Luoyang, Henan, China.
Biol Trace Elem Res. 2024 Dec 21. doi: 10.1007/s12011-024-04496-8.
Selenium (Se) is a trace element that is essential for health. Organic Se created by Se-enriched microorganisms has the characteristics of low toxicity, high bioavailability, and regulation of physiological functions. Here, the regulatory effect of Se-enriched Aspergillus oryzae A02 on the reproductive function of male mice and its potential molecular mechanism was studied. Specifically, twenty-four male mice were randomly divided into a control group and a Se-enriched A. oryzae A02 (Nano-Se) (daily gavage of 0.5 mg/kg, dissolved in saline) for an 8-week experiment. The results showed that Nano-Se intervention did not affect body weight and testicular index, but increased sperm concentration and seminiferous epithelium height in experimental mice, indicating that Nano-Se has the potential to improve the reproductive performance of male mice. Mechanistically, Nano-Se intervention increased the levels of antioxidant-related indicators catalase (CAT) and glutathione peroxidase (GSH-Px) in mouse serum, and increased the relative mRNA expression of GSH-Px, heme oxygenase-1 (HO-1), and NADPH quinine oxidoreductase-1 (NQO-1) in testicular tissues. We identified 9,10,13-trihydroxyoctadecenoic acids (TriHOMEs), stearidonic acid and selenomethionine linked with alpha-linolenic acid metabolism, selenocompound metabolism, folate biosynthesis, ubiquinone, and other terpenoid-quinone biosynthesis and biosynthesis of cofactors. In addition, Nano-Se did not influence the fecal bacterial alpha and beta diversity (P > 0.05), but increased the abundance of the Actinobacteriota and Proteobacteria phyla and the Staphylococcus and Corynebacterium genera, and lowered the abundance of the Bacteroidota phylum and the Lactobacillus and norank_f_Muribaculaceae genera. Nano-Se is considered a novel and promising nutritional regulator to improve reproductive function.
硒（Se）是一种对健康至关重要的微量元素。由富硒微生物产生的有机硒具有低毒性、高生物利用度和调节生理功能的特点。在此，研究了富硒米曲霉A02对雄性小鼠生殖功能的调节作用及其潜在分子机制。具体而言，将24只雄性小鼠随机分为对照组和富硒米曲霉A02（纳米硒）组（每日灌胃0.5 mg/kg，溶于生理盐水），进行为期8周的实验。结果表明，纳米硒干预不影响实验小鼠的体重和睾丸指数，但提高了精子浓度和生精上皮高度，表明纳米硒具有改善雄性小鼠生殖性能的潜力。机制上，纳米硒干预提高了小鼠血清中抗氧化相关指标过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GSH-Px）的水平，并增加了睾丸组织中GSH-Px、血红素加氧酶-1（HO-1）和NADPH醌氧化还原酶-1（NQO-1）的相对mRNA表达。我们鉴定出与α-亚麻酸代谢、硒化合物代谢、叶酸生物合成、泛醌和其他萜类醌生物合成以及辅因子生物合成相关的9,10,13-三羟基十八碳烯酸（TriHOMEs）、十八碳四烯酸和硒代蛋氨酸。此外，纳米硒不影响粪便细菌的α和β多样性（P>0.05），但增加了放线菌门和变形菌门以及葡萄球菌属和棒状杆菌属的丰度，并降低了拟杆菌门以及乳酸杆菌属和未分类_f_毛螺菌科属的丰度。纳米硒被认为是一种新型且有前景的改善生殖功能的营养调节剂。
