Tamaru Erina, Kokubu Daichi, Ushida Yusuke, Itoh Ken
Diet & Well-being Research Institute, KAGOME CO, LTD. 17 Nishitomiyama, Nasushiobara, 329- 2762, Tochigi, Japan.
Department of Vegetable Life Science, Graduate School of Medicine, Hirosaki University, 5 Zaifu-cho, Hirosaki, 036-8562, Aomori, Japan.
BMC Res Notes. 2024 Dec 20;17(1):373. doi: 10.1186/s13104-024-07038-6.
Various plants have been reported to contain compounds that promote the transcriptional activity of Nuclear factor erythroid 2-related factor 2 (Nrf2) to induce a set of xenobiotic detoxifying enzymes, such as NAD(P)H-quinone acceptor oxidoreductase 1 (NQO1), via the antioxidant response element (ARE). While conventional methods for evaluating Nrf2 induction potency include measurement of NQO1 activity, an ARE luciferase reporter assay was recently developed to specifically assess Nrf2 induction potency of compounds of interest. In this study, we compared the abilities of these two assays to evaluate and determine Nrf2 induction potency of plant-derived compounds.
Although the compounds exhibited a high degree of consistency between assays, several compounds did not. These results suggest that although the NQO1 assay can be used as an evaluation method to estimate Nrf2 induction potency of a compound, an ARE luciferase reporter approach may offer greater precision. In summary, inconsistency in Nrf2 induction potency evaluated by the reporter and NQO1 assays of plant-derived compounds, including resveratrol, may be due to a variety of factors that may regulate NQO1 gene expression other than Nrf2 and/or directly modulate NQO1 enzymatic activity or protein levels, with each compound having a different degree of effect on these factors.
据报道，多种植物含有能通过抗氧化反应元件（ARE）促进核因子红细胞2相关因子2（Nrf2）转录活性，从而诱导一系列外源性解毒酶的化合物，如NAD（P）H-醌受体氧化还原酶1（NQO1）。虽然评估Nrf2诱导能力的传统方法包括测量NQO1活性，但最近开发了一种ARE荧光素酶报告基因检测法，以专门评估感兴趣化合物的Nrf2诱导能力。在本研究中，我们比较了这两种检测方法评估和确定植物源化合物Nrf2诱导能力的能力。
尽管这些化合物在两种检测方法之间表现出高度一致性，但仍有几种化合物并非如此。这些结果表明，虽然NQO1检测法可作为评估化合物Nrf2诱导能力的一种方法，但ARE荧光素酶报告基因检测法可能具有更高的准确性。总之，包括白藜芦醇在内的植物源化合物通过报告基因检测法和NQO1检测法评估的Nrf2诱导能力不一致，可能是由于多种因素导致除Nrf2之外还可能调节NQO1基因表达和/或直接调节NQO1酶活性或蛋白质水平，每种化合物对这些因素的影响程度不同。
