Al-Matroushi Abdul Rahman, Shahid Muhammad Shafiq, Khan Jamal, Al-Sadi Abdullah Mohammed
Department of Plant Sciences, College of Agricultural and Marine Sciences, Sultan Qaboos University, Al-Khoud 123, Muscat, Oman.
Cell Mol Biol (Noisy-le-grand). 2024 Nov 27;70(11):101-108. doi: 10.14715/cmb/2024.70.11.15.
Tomato yellow leaf curl virus-Oman (TYLCV-OM), a variant of the Tomato yellow leaf curl virus-Iran (TYLCV-IR) strain, was identified in 2005 as the cause of tomato yellow leaf curl disease (TYLCD) in Oman and is associated with a betasatellite namely as Tomato leaf curl betasatellite (ToLCB). Surveys were carried out from three diverse Governorates of Oman to investigate the correlation between the betasatellite and the virus. The visual assessment and scoring of infected tomato plants in the field revealed that the association of betasatellite with the disease was highest in Sharqia at 77%, followed by Dakhlia at41% and lowest in Batinah at30% . Ten isolates from three distinct regions of Oman were analyzed: two from Al Batinah, two from A'Dakhliah, and six from A'Sharquiah. All TYLCV-OM isolates were identified as variants of the 2005 isolates Al Batinah. However, a new recombinant form of TYLCV-OM, which could impact its virulence or spread, was identified in the Al Batinah region. Mutations observed in the Al Batinah isolates of TYLCV-OM coincided with recombination events involving ToLCOMV. Examination of the intergenic regions (IRs) of TYLCV-OM and ToLCOMV indicated that recombination occurred within the IR. Specifically, TYLCV-OM acquired a segment spanning coordinates 1 to 132 nt from ToLCOMV, which may influence its genetic diversity. The implications of these findings for the evolutionary dynamics of the begomovirus complex associated with yellow leaf curl disease are discussed. Inoculation of infectious construct of TYLCV-OM alone or with ToLCB resulted in severe leaf curl symptoms but leaf yellowing was more pronounced in the presence of ToLCB. Real-time quantitative data showed that TYLCV-OM was accumulated to higher level in the presence of betasatellite.
番茄黄化曲叶病毒-阿曼株（TYLCV-OM）是番茄黄化曲叶病毒-伊朗株（TYLCV-IR）的一个变种，于2005年被确定为阿曼番茄黄化曲叶病（TYLCD）的病因，并且与一种名为番茄曲叶β卫星（ToLCB）的β卫星有关。在阿曼三个不同的省份进行了调查，以研究该β卫星与病毒之间的相关性。对田间受感染番茄植株的目视评估和评分显示，β卫星与该病的关联在Sharqia最高，为77%，其次是Dakhlia，为41%，在Batinah最低，为30%。对来自阿曼三个不同地区的10个分离株进行了分析：两个来自Al Batinah，两个来自A'Dakhliah，六个来自A'Sharquiah。所有TYLCV-OM分离株均被鉴定为2005年Al Batinah分离株的变种。然而，在Al Batinah地区发现了一种新的TYLCV-OM重组形式，它可能会影响其毒力或传播。在TYLCV-OM的Al Batinah分离株中观察到的突变与涉及ToLCOMV的重组事件一致。对TYLCV-OM和ToLCOMV的基因间隔区（IRs）的检查表明，重组发生在IR内。具体而言，TYLCV-OM从ToLCOMV获得了一个跨越坐标1至132 nt的片段，这可能会影响其遗传多样性。讨论了这些发现对与黄化曲叶病相关的双生病毒复合体进化动态的影响。单独接种TYLCV-OM的感染性构建体或与ToLCB一起接种会导致严重的叶片卷曲症状，但在存在ToLCB的情况下叶片黄化更明显。实时定量数据显示，在存在β卫星的情况下，TYLCV-OM积累到更高水平。
