Chand Gulab, Zunjare Rajkumar Uttamrao, Hossain Firoz, Muthusamy Vignesh, Katral Ashvinkumar, Chauhan Priyanka, Rathore Aayushi Singh, Mishra Subhra Jyotshna
ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India.
Mol Biol Rep. 2024 Dec 21;52(1):72. doi: 10.1007/s11033-024-10170-y.
The role of the silkless1 (sk1) gene in developing silkless baby corn, a distinctive trait in maize has been investigated. So far, no sk1 gene-specific marker has been available for accelerated development of silkless baby corn hybrids.
METHODS & RESULTS: We developed sk1 gene-based markers and validated them in backcross (BC) and F segregating generations, revealing a polymorphic marker corresponding to a silkless phenotype. Among all the developed markers, the MGU_sk1-1 marker (141 bp size in sk1-mutant allele) validated its association with the silkless trait. The chi square (χ) analysis revealed that the segregation of alleles Sk1Sk1 (219): Sk1sk1 (414): sk1sk1 (188) is as per the Mendelian ratio (1:2:1) in the F population. In 821 F plants, we found a 3:1 phenotypic ratio among the population with silked (633) and silkless (188) plants. We studied 349 BC plants and found a 1:1 phenotypic ratio between silked and silkless plants, as well as a genotypic ratio (1:1) between heterozygotes (Sk1sk1) and homozygous recessives (sk1sk1). Additionally, double mutants (sk1sk1/ts1ts1 and sk1sk1/ts2ts2) were identified using the sk1-based marker.
This study is the first to show the practical use of sk1 gene in marker-assisted selection (MAS). This research also reports the development of novel genetic resources for innovative silkless baby corn breeding.
已对无花丝1（sk1）基因在培育无花丝甜玉米（玉米的一种独特性状）中的作用进行了研究。到目前为止，尚无sk1基因特异性标记可用于加速无花丝甜玉米杂交种的培育。
我们开发了基于sk1基因的标记，并在回交（BC）和F分离世代中对其进行了验证，揭示了一个与无花丝表型相对应的多态性标记。在所有开发的标记中，MGU_sk1 - 1标记（sk1突变等位基因大小为141 bp）验证了其与无花丝性状的关联。卡方（χ）分析表明，在F群体中，等位基因Sk1Sk1（219）：Sk1sk1（414）：sk1sk1（188）的分离符合孟德尔比例（1:2:1）。在821株F植株中，我们发现有花丝（633株）和无花丝（188株）植株的群体中表型比例为3:1。我们研究了349株BC植株，发现有花丝和无花丝植株之间的表型比例为1:1，杂合子（Sk1sk1）和纯合隐性个体（sk1sk1）之间的基因型比例也为1:1。此外，使用基于sk1的标记鉴定出了双突变体（sk1sk1/ts1ts1和sk1sk1/ts2ts2）。
本研究首次展示了sk1基因在标记辅助选择（MAS）中的实际应用。本研究还报道了用于创新型无花丝甜玉米育种的新型遗传资源的开发。
