Cai Yanting, Li Yue, Wang Yuanyuan, Xu Yihan, Chen Tianyan, Xue Ruisong, Liu Yanmei, Chen Wei, Yang Xiurong, Liu Zhen, Bao Xingfu, Huang Zhenzhen
Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, School and Hospital of Stomatology, Jilin University, 130021, Changchun, China.
College of Chemistry, Jilin University, 130012, Changchun, China.
Biosens Bioelectron. 2025 Mar 1;271:117078. doi: 10.1016/j.bios.2024.117078. Epub 2024 Dec 17.
Acetylcholinesterase (AChE) and AChE inhibitors play critical roles in the early diagnosis and treatment of Alzheimer's disease (AD). Herein, a fluorescence/colorimetry/smartphone triple-mode sensing platform was constructed for both AChE activity monitoring and AChE inhibitor screening by exploring a Cu (I) compound, CuI (SR) (R = CHCHNH), as a fluorescent probe. In comparison of most other fluorescent probes, CuI (SR) presented exceptional stability against pH, temperature, UV irradiation, redox agents, and metal ions, as well as good recyclability due to its unique chemical structure. We further found the fluorescence emission of CuI (SR) could be quenched by MnO nanosheet (NS) via inner filter effect, and restored by thiocholine (TCh) generated from the hydrolysis of acetylthiocholine iodide (ATCh) in the catalysis of AChE. On this basis, a fluorescence "turn-on" assay was developed for monitoring AChE activity with a detection limit of 0.03 U/L and a detection range of 0.25-50 U/L. This method demonstrates great potential for real-time detection of AChE activity in biological samples and screening of AChE inhibitors obtained from herbal extracts as anti-AD agents. Additionally, CuI (SR)/MnO NS sensing system also exhibited a color change from brown to colorless as the increasing AChE activity, which allowed the colorimetric and smartphone detection of AChE activity.
乙酰胆碱酯酶（AChE）及其抑制剂在阿尔茨海默病（AD）的早期诊断和治疗中发挥着关键作用。在此，通过探索一种铜（I）化合物CuI（SR）（R = CHCHNH）作为荧光探针，构建了一种用于监测AChE活性和筛选AChE抑制剂的荧光/比色法/智能手机三模式传感平台。与大多数其他荧光探针相比，CuI（SR）对pH、温度、紫外线照射、氧化还原试剂和金属离子具有出色的稳定性，并且由于其独特的化学结构而具有良好的可回收性。我们进一步发现，MnO纳米片（NS）可通过内滤效应淬灭CuI（SR）的荧光发射，而在AChE催化下，碘化硫代乙酰胆碱（ATCh）水解产生的硫代胆碱（TCh）可恢复其荧光。在此基础上，开发了一种荧光“开启”分析法用于监测AChE活性，检测限为0.03 U/L，检测范围为0.25 - 50 U/L。该方法在生物样品中实时检测AChE活性以及筛选从草药提取物中获得的作为抗AD药物的AChE抑制剂方面显示出巨大潜力。此外，随着AChE活性增加，CuI（SR）/MnO NS传感系统还表现出从棕色到无色的颜色变化，这使得可以通过比色法和智能手机检测AChE活性。
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