Di Moyan, Gong Xinbao, Zhu Yanyun, Guo Shibao, Pan Zhihao, Li Mengxian, Wu Zhuoya, Zhang Wei, Liu Xuelan, Liu Ya, Li Yunsheng, Li Jian, Fang Fugui
Department of Animal Veterinary Science, College of Animal Science and Technology, Anhui Agricultural University, 130 Changjiang West Road, Hefei, Anhui 230036, China.
Department of Animal Veterinary Science, College of Animal Science and Technology, Anhui Agricultural University, 130 Changjiang West Road, Hefei, Anhui 230036, China; Anhui Province Key Laboratory of Local Livestock and Poultry, Genetical Resource Conservation and Breeding, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China.
Domest Anim Endocrinol. 2025 Apr;91:106908. doi: 10.1016/j.domaniend.2024.106908. Epub 2024 Dec 15.
Gonadotropin-releasing hormone (GnRH) vaccines have been widely used to effectively inhibit gonadal development and reproductive function. To improve the immunogenicity of GnRH, we developed and evaluated the effects of GnRH6-kisspeptin-CRM197 immunization on the reproductive function in male goats. Thirty 3-month-old male goats (n = 30) were randomly assigned to control, surgical, and immunized groups. The immunized group received a 2 mL injection of the GnRH6-kisspeptin-CRM197 with a booster administered four weeks later. The control group was administered a white oil adjuvant. Blood samples were collected at regular intervals, and at week 20, the animals were euthanized for tissue collection. Serum antibody titers and testosterone levels were measured using ELISA and CLIA, respectively. Testicular parameters and histology were evaluated. The mRNA levels of reproductive-related genes in the HPG axis were measured using RT-qPCR. The results showed that the immunized goats had significantly increased serum GnRH and kisspeptin antibodies (P < 0.05) but decreased testosterone concentrations (P < 0.05) compared to the control group. Testicular size and histology were significantly affected in the immunized group, with notable reductions in testicular weight and dimensions (P < 0.01), and evidence of vacuolar degeneration and suppressed sperm production. The mRNA levels of FSHβ and LHβ in the pituitary, as well as FSHR, LHR, 3βHSD, and 17βHSD in the testis, were significantly lower in the immunized group compared to controls (P < 0.05). These findings suggest that GnRH6-kisspeptin-CRM197 is a safe antigen and a promising immunocastration vaccine with enhanced efficacy.
促性腺激素释放激素（GnRH）疫苗已被广泛用于有效抑制性腺发育和生殖功能。为提高GnRH的免疫原性，我们研发并评估了GnRH6- kisspeptin - CRM197免疫对雄性山羊生殖功能的影响。30只3月龄雄性山羊（n = 30）被随机分为对照组、手术组和免疫组。免疫组注射2 mL GnRH6- kisspeptin - CRM197，四周后进行加强免疫。对照组注射白油佐剂。定期采集血样，在第20周时对动物实施安乐死以收集组织。分别使用酶联免疫吸附测定法（ELISA）和化学发光免疫分析法（CLIA）检测血清抗体滴度和睾酮水平。评估睾丸参数和组织学情况。使用逆转录定量聚合酶链反应（RT-qPCR）测定下丘脑-垂体-性腺（HPG）轴中生殖相关基因的mRNA水平。结果显示，与对照组相比，免疫山羊的血清GnRH和kisspeptin抗体显著升高（P < 0.05），但睾酮浓度降低（P < 0.05）。免疫组的睾丸大小和组织学受到显著影响，睾丸重量和尺寸明显减小（P < 0.01），并有空泡变性和精子生成受抑制的迹象。与对照组相比，免疫组垂体中促卵泡生成素β（FSHβ）和促黄体生成素β（LHβ）以及睾丸中促卵泡生成素受体（FSHR）、促黄体生成素受体（LHR）、3β-羟基类固醇脱氢酶（3βHSD）和17β-羟基类固醇脱氢酶（17βHSD）的mRNA水平显著降低（P < 0.05）。这些发现表明，GnRH6- kisspeptin - CRM197是一种安全的抗原，是一种有前景的、效力增强的免疫去势疫苗。
