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One-step generation of prime-edited transgene-free rice.

作者信息

Lu Yu, Naren Tuya, Qiao Dexin, Wang Junya, Lyu Tiantian, Cao Zhenghong, Sun Wei, Ji Xuejiao, Chen Qi-Jun, Jiang Linjian

机构信息

State Key Laboratory of Plant Environmental Resilience, College of Biological Sciences, China Agricultural University, Beijing 100193, China.

Department of Plant Biosecurity, Key Laboratory of Surveillance and Management for Plant Quarantine Pests, Ministry of Agriculture and Rural Affairs, College of Plant Protection, China Agricultural University, Beijing 100193, China; State Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, China Agricultural University, Beijing 100193, China.

出版信息

Plant Commun. 2025 Apr 14;6(4):101227. doi: 10.1016/j.xplc.2024.101227. Epub 2024 Dec 20.

DOI:10.1016/j.xplc.2024.101227
PMID:39709522
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12010367/
Abstract
摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/507b/12010367/10a0b0197da0/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/507b/12010367/10a0b0197da0/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/507b/12010367/10a0b0197da0/gr1.jpg

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本文引用的文献

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Cas9-PE: a robust multiplex gene editing tool for simultaneous precise editing and site-specific random mutation in rice.Cas9-PE：一种用于水稻同时进行精确编辑和位点特异性随机突变的强大多重基因编辑工具。
Trends Biotechnol. 2025 Feb;43(2):433-446. doi: 10.1016/j.tibtech.2024.10.012. Epub 2024 Nov 12.
2
PE6c greatly enhances prime editing in transgenic rice plants.PE6c 极大地增强了转基因水稻中的 Prime 编辑。
J Integr Plant Biol. 2024 Sep;66(9):1864-1870. doi: 10.1111/jipb.13738. Epub 2024 Jul 9.
3
Transgene-free genome editing of vegetatively propagated and perennial plant species in the T0 generation via a co-editing strategy.
通过共编辑策略在 T0 代中对营养繁殖和多年生植物物种进行无转基因基因组编辑。
Nat Plants. 2023 Oct;9(10):1591-1597. doi: 10.1038/s41477-023-01520-y. Epub 2023 Sep 18.
4
Phage-assisted evolution and protein engineering yield compact, efficient prime editors.噬菌体辅助进化和蛋白质工程产生了紧凑、高效的 Prime 编辑器。
Cell. 2023 Aug 31;186(18):3983-4002.e26. doi: 10.1016/j.cell.2023.07.039.
5
Efficient and versatile multiplex prime editing in hexaploid wheat.六倍体小麦中高效且多功能的多重 Prime 编辑。
Genome Biol. 2023 Jun 29;24(1):156. doi: 10.1186/s13059-023-02990-1.
6
Prime editing for precise and highly versatile genome manipulation.碱基编辑技术实现精准且多功能的基因组编辑。
Nat Rev Genet. 2023 Mar;24(3):161-177. doi: 10.1038/s41576-022-00541-1. Epub 2022 Nov 7.
7
Search-and-replace genome editing without double-strand breaks or donor DNA.无双链断裂或供体 DNA 的搜索和替换基因组编辑。
Nature. 2019 Dec;576(7785):149-157. doi: 10.1038/s41586-019-1711-4. Epub 2019 Oct 21.
8
A Novel Ternary Vector System United with Morphogenic Genes Enhances CRISPR/Cas Delivery in Maize.一种新型三元载体系统联合形态发生基因增强了玉米中的 CRISPR/Cas 递送。
Plant Physiol. 2019 Dec;181(4):1441-1448. doi: 10.1104/pp.19.00767. Epub 2019 Sep 26.
9
Generation of herbicide tolerance traits and a new selectable marker in wheat using base editing.利用碱基编辑技术在小麦中生成除草剂耐受特性和新的可选择标记。
Nat Plants. 2019 May;5(5):480-485. doi: 10.1038/s41477-019-0405-0. Epub 2019 Apr 15.
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Transgene-Free Genome Editing in Tomato and Potato Plants Using -Mediated Delivery of a CRISPR/Cas9 Cytidine Base Editor.利用 - 介导的 CRISPR/Cas9 胞嘧啶碱基编辑器在番茄和马铃薯植物中进行无转基因基因组编辑。
Int J Mol Sci. 2019 Jan 18;20(2):402. doi: 10.3390/ijms20020402.