Chen Yiliang, Tang Siyuan, Hameed Muhammad Salman, Xu Xiaolong, Wang Qi, Chen Qian, Yan Jufen, Zhang Kui, Han Xinya
School of Chemistry and Chemical Engineering, Anhui University of Technology, Ma'anshan 243032, PR China.
State Key Laboratory of Green Pesticide, International Joint Research Center for Intelligent Biosensor Technology and Health, Central China Normal University, Wuhan 430079, PR China.
Bioorg Chem. 2025 Jan;154:108061. doi: 10.1016/j.bioorg.2024.108061. Epub 2024 Dec 11.
Peroxynitrite (ONOO) and viscosity are critical indicators of lysosome functionality, intimately linked to numerous diseases' pathophysiological processes. Hence, creating reliable analytical techniques to observe fluctuations in lysosomal ONOO and viscosity is highly important. This study presents the development of a novel naphthalimide-based fluorescent probe, Nap-Cy, specifically designed to target lysosomes and simultaneously detect both ONOO and viscosity. Nap-Cy displayed a near-infrared fluorescence "turn-on" response to viscosity (ranging from 1.0 to 1410 cp) with an approximately 400-fold increase in intensity. At the same time, it functioned as a ratiometric probe with high sensitivity for detecting ONOO, featuring a quick response time of approximately 10 min, a low detection limit of 42 nM, a broad pH range (5-11), and excellent selectivity for ONOO over other chemical and biological species. Additionally, Nap-Cy was successfully applied for fluorescence imaging to monitor ONOO and viscosity variations in SH-SY5Y cells and zebrafish across multiple channels. This research introduces a valuable molecular probe for investigating the biological functions and interactions of ONOO and viscosity within lysosomes.
