Wang Run-Xi, Zhou Hong-Bing, Gao Jia-Xing, Bai Wan-Fu, Wang Jia, Bai Ying-Chun, Jiang Shu-Yuan, Chang Hong, Shi Song-Li
Department of Pharmacy, Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou 014040, China.
Department of Pharmacy, Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou 014040, China; Institute of Bioactive Substance and Function of Chinese Materia Medica and Mongolian Medicine, Baotou Medical College, Inner Mongolia University of Science and Technology, Baotou 014040, China.
Phytomedicine. 2025 Jan;136:156335. doi: 10.1016/j.phymed.2024.156335. Epub 2024 Dec 17.
Renal fibrosis (RF) is an inevitable consequence of multiple manifestations of progressive chronic kidney diseases (CKDs). Mechanism of Amygdalus mongolica (Maxim.) in the treatment of RF needs further investigation.
The study further investigated the potential mechanism of A. mongolica in the treatment of RF.
A rat model of RF was induced by unilateral ureteral obstruction (UUO), followed by treatment with varying dosages of A. mongolica oil for 4 weeks. Body weight was measured weekly. We detected serum levels of interleukin (IL)-6, IL-1β, type Ⅲ procollagen (Col-Ⅲ), type IV collagen (Col-Ⅳ), laminin (LN), hyaluronidase (HA), and tissue levels of albumin (ALB), blood urea nitrogen (BUN), creatinine (Cre), superoxide dismutase (SOD), malondialdehyde (MDA), and hydroxyproline (HYP). Shotgun metagenomics analyzed the composition of the intestinal microbiota. High-performance liquid chromatography coupled with a quadrupole-exactive mass spectrometer (HPLC-Q-Exactive-MS) monitored changes in metabolite levels in serum and gut. Multiple reaction monitoring-mass spectrometry (MRM-MS) determined the levels of amino acids in serum.
A. mongolica oil significantly alleviated indicators related to RF (p < 0.05). A. mongolica oil reduced the ratio of Firmicutes to Bacteroidetes and restored the balance of intestinal microbiota in rats with RF. A. mongolica oil modulated levels of metabolites in gut content and serum. It regulated 11 metabolic pathways including arachidonic acid metabolism. Targeted metabolomics of amino acids showed that 17 amino acids were significantly changed by A. mongolica oil, including L-glycine, L-serine and L-glutamine.
A. mongolica oil regulates intestinal microbiota and metabolites, restoring amino acid metabolism to treat RF.
肾纤维化（RF）是进行性慢性肾脏病（CKD）多种表现的必然结果。蒙古扁桃（Maxim.）治疗RF的机制尚需进一步研究。
本研究进一步探究蒙古扁桃治疗RF的潜在机制。
通过单侧输尿管梗阻（UUO）诱导建立RF大鼠模型，随后用不同剂量的蒙古扁桃油治疗4周。每周测量体重。检测血清白细胞介素（IL）-6、IL-1β、Ⅲ型前胶原（Col-Ⅲ）、Ⅳ型胶原（Col-Ⅳ）、层粘连蛋白（LN）、透明质酸酶（HA）水平，以及组织白蛋白（ALB）、血尿素氮（BUN）、肌酐（Cre）、超氧化物歧化酶（SOD）、丙二醛（MDA）和羟脯氨酸（HYP）水平。鸟枪法宏基因组学分析肠道微生物群的组成。高效液相色谱-四极杆-高分辨质谱联用仪（HPLC-Q-Exactive-MS）监测血清和肠道中代谢物水平的变化。多反应监测质谱（MRM-MS）测定血清中氨基酸水平。
蒙古扁桃油显著改善了与RF相关的指标（p<0.05）。蒙古扁桃油降低了RF大鼠中厚壁菌门与拟杆菌门的比例，恢复了肠道微生物群的平衡。蒙古扁桃油调节了肠道内容物和血清中的代谢物水平。它调控了包括花生四烯酸代谢在内的11条代谢途径。氨基酸靶向代谢组学显示，17种氨基酸因蒙古扁桃油而发生显著变化，包括L-甘氨酸、L-丝氨酸和L-谷氨酰胺。
蒙古扁桃油通过调节肠道微生物群和代谢物，恢复氨基酸代谢来治疗RF。
