Corin Aaron, Nora Elphège P, Ramani Vijay
Tetrad Graduate Program, University of California, San Francisco, San Francisco, CA, USA; Gladstone Institute for Data Science and Biotechnology, Gladstone Institutes, San Francisco, CA, USA; Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA, USA.
Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA, USA; Cardiovascular Research Institute, University of California, San Francisco, San Francisco, CA, USA; Chan-Zuckerberg Biohub, San Francisco, CA, USA.
Curr Opin Genet Dev. 2025 Feb;90:102298. doi: 10.1016/j.gde.2024.102298. Epub 2024 Dec 21.
CCCTC-binding factor (CTCF) is a key regulator of 3D genome organization and transcriptional activity. Beyond its well-characterized role in facilitating cohesin-mediated loop extrusion, CTCF exhibits several cohesin-independent activities relevant to chromatin structure and various nuclear processes. These functions include patterning of nucleosome arrangement and chromatin accessibility through interactions with ATP-dependent chromatin remodelers. In addition to influencing transcription, DNA replication, and DNA repair in ways that are separable from its role in loop extrusion, CTCF also interacts with RNA and contributes to RNA splicing and condensation of transcriptional activators. Here, we review recent insight into cohesin-independent activities of CTCF, highlighting its multifaceted roles in chromatin biology and transcriptional regulation.
CCCTC结合因子（CTCF）是三维基因组组织和转录活性的关键调节因子。除了在促进黏连蛋白介导的环挤压中具有明确的作用外，CTCF还表现出几种与染色质结构和各种核过程相关的不依赖黏连蛋白的活性。这些功能包括通过与ATP依赖的染色质重塑因子相互作用来调控核小体排列模式和染色质可及性。除了以与其在环挤压中的作用可分离的方式影响转录、DNA复制和DNA修复外，CTCF还与RNA相互作用，并有助于RNA剪接和转录激活因子的凝聚。在这里，我们综述了对CTCF不依赖黏连蛋白活性的最新见解，强调了其在染色质生物学和转录调控中的多方面作用。
