通过调节反刍动物瘤胃微生物群减少甲烷排放的膳食抑制剂的Meta分析。

A Meta-Analysis of Dietary Inhibitors for Reducing Methane Emissions via Modulating Rumen Microbiota in Ruminants.

作者信息

Ma Guiling, Jin Wei, Zhang Yu, Gai Yang, Tang Weixuan, Guo Lu, Azzaz Hossam H, Ghaffari Morteza H, Gu Zhaobing, Mao Shengyong, Chen Yanting

机构信息

Institute of Ensiling and Processing of Grass, College of Agro-grassland Science, Nanjing Agricultural University, Nanjing, China; National Center for International Research on Animal Gut Nutrition, Jingsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, Nanjing Agricultural University, Nanjing, China; College of Animal Science & Technology, Nanjing Agricultural University, Nanjing, China.

National Center for International Research on Animal Gut Nutrition, Jingsu Key Laboratory of Gastrointestinal Nutrition and Animal Health, Nanjing Agricultural University, Nanjing, China; College of Animal Science & Technology, Nanjing Agricultural University, Nanjing, China.

出版信息

J Nutr. 2025 Feb;155(2):402-412. doi: 10.1016/j.tjnut.2024.12.011. Epub 2024 Dec 20.

DOI:10.1016/j.tjnut.2024.12.011

PMID:39710134

Abstract

BACKGROUND

Rumen methane emissions (RMEs) significantly contribute to global greenhouse gas emissions, underscoring the essentials to identify effective inhibitors for RME mitigation. Despite various inhibitors shown potential in reducing RME by modulating rumen microbes, their impacts include considerable variations and inconsistency.

OBJECTIVES

We aimed to quantitatively assess the impacts of various methane inhibitors on RME, rumen microbial abundance, and fermentation in ruminants. Additionally, the relationships between microbial abundance and RME were examined through meta-regressions.

METHODS

Meta-analysis and meta-regression were conducted to assess the impacts of methane inhibitions, including 3-nitrooxypropanol, ionophores, nitrate, triglycerides, phytochemicals, and co-inhibitors, on RME and rumen microbiota in beef, dairy cattle, and sheep.

RESULTS

Analyses of 922 datasets from 274 experiments revealed that inhibitors, except ionophores (P = 0.43), significantly reduced RME, with co-inhibitors displaying the highest efficacy (standardized mean difference -2.1, P < 0.01). Inhibitors' effects were more pronounced in sheep relative to beef and dairy cattle. Inhibitors decreased the abundance of ciliates and methanogens, with positive correlations observed between Dasytrichidae (P = 0.05), Entodinomorphs (P ≤ 0.001), Methanobacteriale (P = 0.001), and fungi (P < 0.01) with RME. Among inhibitors, triglycerides exhibited simultaneous reduction in methanogen, ciliate, and fungal abundances. 3-Nitrooxypropanol and triglycerides increased H in the rumen whereas reducing the acetate-propionate ratio, especially in beef. The H emission was negatively correlated (P < 0.01) and acetate-to-propionate ratio was positively correlated (P < 0.001) with RME.

CONCLUSIONS

Microbes, including Dasytrichidae, Entodinomorphs, Methanobacteriale, and fungi, significantly attribute to RME, and co-inhibitors have the highest efficacy in limiting RME and reducing microbial abundances. This study underscores the roles of both host and microbiota in modulating the inhibitor efficacy in RME, informing the refinement of rumen additives to mitigate RME from meat and milk production.

摘要

背景

瘤胃甲烷排放对全球温室气体排放有显著贡献，这凸显了识别有效抑制剂以减轻瘤胃甲烷排放的必要性。尽管各种抑制剂通过调节瘤胃微生物显示出减少瘤胃甲烷排放的潜力，但其影响存在相当大的差异和不一致性。

目的

我们旨在定量评估各种甲烷抑制剂对反刍动物瘤胃甲烷排放、瘤胃微生物丰度和发酵的影响。此外，通过meta回归分析研究微生物丰度与瘤胃甲烷排放之间的关系。

方法

进行meta分析和meta回归，以评估包括3-硝基氧丙醇、离子载体、硝酸盐、甘油三酯、植物化学物质和联合抑制剂在内的甲烷抑制剂对肉牛、奶牛和绵羊瘤胃甲烷排放和瘤胃微生物群的影响。

结果

对来自274项实验的922个数据集的分析表明，除离子载体外（P = 0.43），其他抑制剂均显著降低了瘤胃甲烷排放，联合抑制剂的效果最为显著（标准化平均差-2.1，P < 0.01）。与肉牛和奶牛相比，抑制剂对绵羊的影响更为明显。抑制剂降低了纤毛虫和产甲烷菌的丰度，发现多泡球虫科（P = 0.05）、内毛虫属（P ≤ 0.001）、甲烷杆菌目（P = 0.001）和真菌（P < 0.01）与瘤胃甲烷排放呈正相关。在抑制剂中，甘油三酯同时降低了产甲烷菌、纤毛虫和真菌的丰度。3-硝基氧丙醇和甘油三酯增加了瘤胃中的氢气含量，同时降低了乙酸盐与丙酸盐的比例，尤其是在肉牛中。氢气排放与瘤胃甲烷排放呈负相关（P < 0.01），乙酸盐与丙酸盐的比例与瘤胃甲烷排放呈正相关（P < 0.001）。