Attenuation of Chronic Oxidative Stress-Induced Testicular and Epididymal Dysfunction by Oral Intake of Lepidium meyenii in New Zealand Rabbits.

Lepidium meyenii (Maca) is a plant that has nutritional benefits and increases the effectiveness of male reproduction. In this study, oxidative stress-exposed New Zealand rabbits were used to assess the ameliorative effects of daily Maca ingestion on testicular and epididymal tissues as well as the quality of fresh and frozen/thawed sperm. Twenty-four 40-week-old, healthy New Zealand white male rabbits were divided into four groups. The first group consumed tap water and served as a control. The second group was given 300 mg of Maca daily in capsules. The third group drank water containing hydrogen peroxide (HO) at a concentration of 1%. Finally, the fourth group consumed HO and Maca daily. The ejaculate volume, sperm concentration, vitality, motility and velocity of the HO group were considerably lower than those of the other groups. Frozen/thawed spermatozoa showed more dramatic decreases in motility and velocity as a result of HO consumption. The plasma concentrations of testosterone and total antioxidant capacity were also lowest in the HO-treated rabbits, while malondialdehyde levels were highest. Exposure to HO increased collagen deposition between ST and epididymal ducts which induced testicular and epididymis fibrosis. In addition, the spermatogenic and epididymal epithelial cells exhibited signs of apoptosis, degeneration, vacuolation and a reduction in height. Maca intake attenuated most of the damaging effects of HO ingestion-induced oxidative stress. Furthermore, HO-treated rabbits had modest nuclear androgen receptor positivity, unlike those in the Maca group. The number of Leydig cells significantly increased with daily Maca intake. In conclusion, daily intake of Maca improved reproductive performance and mitigated the damaging effects of oxidative stress on testicular and epididymal functions in New Zealand rabbits.