慢性阻塞性肺疾病急性加重期的动态微生物变化

Dynamic microbial changes in exacerbation of chronic obstructive pulmonary disease.

作者信息

Choi Yong Jun, Park Hye Jung, Kim Chi Young, Choi Aeri, Cho Jae Hwa, Byun Min Kwang

机构信息

Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Gangnam Severance Hospital, Yonsei University College of Medicine, Seoul, Republic of Korea.

出版信息

Front Microbiol. 2024 Dec 6;15:1507090. doi: 10.3389/fmicb.2024.1507090. eCollection 2024.

DOI:10.3389/fmicb.2024.1507090

PMID:39712895

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11659282/

Abstract

BACKGROUND

Microbial profiles in patients with chronic obstructive pulmonary disease (COPD) provide insights for predicting, preventing, and treating exacerbations. This study aimed to analyze the impact of microbial diversity and spectrum on COPD exacerbation.

METHODS

From November 1, 2018, to May 31, 2023, we prospectively enrolled patients with stable disease (SD) and exacerbation of COPD (ECOPD). Sputum samples were collected for microbiome DNA sequencing, and amplicon sequence variants were analyzed.

RESULTS

We collected sputum samples from 38 patients: 17 samples from patients with SD and samples from patients with ECOPD at two time points-during exacerbation (AE-1: 21 samples) and again during stabilization after 2 weeks of treatment (AE-2: 17 samples). Alpha diversity indices, specifically observed feature count and Fisher's alpha index, were significantly higher in SD (133.0 [98.0-145.0]; 17.1 [12.7-19.6]) compared to AE-1 (88.0 [72.0-125.0],  = 0.025; 10.9 [8.5-16.1],  = 0.031). The SD showed significantly higher abundances of (linear discriminant analysis [LDA] 4.996, adj. = 0.021), (LDA 3.688, adj. = 0.047), and (LDA 3.379, adj. = 0.039) at the genus level compared to AE-1. At the species level, (LDA 5.074, adj. = 0.010) and (LDA 4.467, adj.  = 0.011) were more abundant in SD. Hub genera in the microbial network included , , , , and in SD and , , , , and in AE-1.

CONCLUSION

COPD exacerbations are linked to changes in specific strains of normal flora. Maintaining microbial diversity and balance within the microbial network is critical for preventing and managing COPD exacerbations.

摘要

背景

慢性阻塞性肺疾病（COPD）患者的微生物谱为预测、预防和治疗病情加重提供了依据。本研究旨在分析微生物多样性和谱对COPD病情加重的影响。

方法

从2018年11月1日至2023年5月31日，我们前瞻性纳入了病情稳定（SD）和COPD急性加重（ECOPD）的患者。收集痰液样本进行微生物组DNA测序，并分析扩增子序列变异。

结果

我们收集了38例患者的痰液样本：17例来自SD患者，以及ECOPD患者在两个时间点的样本——急性加重期（AE-1：21例样本）和治疗2周后病情稳定期（AE-2：17例样本）。与AE-1相比，SD组的α多样性指数，特别是观察到的特征数和费舍尔α指数显著更高（133.0 [98.0 - 145.0]；17.1 [12.7 - 19.6]），而AE-1为（88.0 [72.0 - 125.0]，P = 0.025；10.9 [8.5 - 16.1]，P = 0.031）。在属水平上，与AE-1相比，SD组的[具体菌属1]（线性判别分析[LDA] 4.996，校正P = 0.021）、[具体菌属2]（LDA 3.688，校正P = 0.047）和[具体菌属3]（LDA 3.379，校正P = 0.039）丰度显著更高。在种水平上，[具体菌种1]（LDA 5.074，校正P = 0.010）和[具体菌种2]（LDA 4.467，校正P = 0.011）在SD组中更为丰富。微生物网络中的核心菌属在SD组包括[菌属1]、[菌属2]、[菌属3]、[菌属4]和[菌属5]，在AE-1组包括[菌属6]、[菌属7]、[菌属8]、[菌属9]和[菌属10]。