Bakri Husna Hazirah, Syed Abdul Rahman Syarifah Nur, Dol Bakri Zarith Safinaz, Munadziroh Elly, Wan Harun Wan Himratul Aznita
Department of Oral & Craniofacial Sciences, Faculty of Dentistry, Universiti Malaya, Kuala Lumpur, Malaysia.
Department of Dental Material, Faculty of Dental Medicine, Airlangga University, Surabaya, East Java, Indonesia.
PeerJ. 2024 Dec 18;12:e18751. doi: 10.7717/peerj.18751. eCollection 2024.
is widely recognised as a periodontal pathogen. In recent years, there has been growing interest in the use of medicinal plant extracts as alternative treatments for periodontitis to combat the emergence of antibiotic-resistant bacteria. L. Pers has been traditionally used to treat various ailments, including oral bacterial infections. However, the antimicrobial potential of extracts against the periodontal pathogen remains unexplored. Hence, the aim of this study was to investigate the antimicrobial activity of extracts against .
The antimicrobial activity of extracts (crude methanol, hexane and chloroform fractionated extracts) against was evaluated using the well diffusion method. Additionally, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined. Biofilm biomass assessment and live/dead cell viability assays were performed to analyse the effect of . extracts. Ultrastructural morphological changes in cells were determined using field emission scanning electron microscopy (FE-SEM).
It was found that was susceptible to . extracts, with the chloroform fractionated extract exhibiting the highest inhibition zones. The MIC and MBC of chloroform fractionated extract were determined to be 6.25 mg/mL which substantially reduced biofilm biomass. Live/dead cell viability assays showed the highest percentage of dead cells after 48 h of incubation. FE-SEM confirmed that the chloroform fractionated extract effectively damaged the bacterial cell wall and altered the ultrastructural morphology of .
The results indicated that extracts of . has the potential to be used as an alternative treatment in addition to conventional periodontal therapies.
[病原体名称]被广泛认为是一种牙周病原体。近年来，人们越来越关注使用药用植物提取物作为牙周炎的替代治疗方法，以对抗抗生素耐药菌的出现。[植物名称]长期以来一直被用于治疗各种疾病，包括口腔细菌感染。然而，[植物名称]提取物对牙周病原体的抗菌潜力仍未得到探索。因此，本研究的目的是调查[植物名称]提取物对[病原体名称]的抗菌活性。
采用滤纸片扩散法评估[植物名称]提取物（粗甲醇提取物、己烷和氯仿分级提取物）对[病原体名称]的抗菌活性。此外，还测定了最低抑菌浓度（MIC）和最低杀菌浓度（MBC）。进行生物膜生物量评估和活/死细胞活力测定，以分析[植物名称]提取物的作用。使用场发射扫描电子显微镜（FE-SEM）确定[病原体名称]细胞的超微结构形态变化。
发现[病原体名称]对[植物名称]提取物敏感，氯仿分级提取物表现出最高的抑菌圈。氯仿分级提取物的MIC和MBC测定为6.25mg/mL，这显著降低了[病原体名称]生物膜生物量。活/死细胞活力测定显示，孵育48小时后，死亡的[病原体名称]细胞百分比最高。FE-SEM证实，氯仿分级提取物有效地破坏了细菌细胞壁，并改变了[病原体名称]的超微结构形态。
结果表明，[植物名称]提取物除了可作为传统牙周治疗方法外，还具有作为替代治疗方法的潜力。
