Beizman-Magen Yana, Orevi Tomer, Kashtan Nadav
Institute of Environmental Sciences, Department of Plant Pathology and Microbiology, Robert H. Smith Faculty of Agriculture, Food, and Environment, Hebrew University, Rehovot, Israel.
Appl Environ Microbiol. 2025 Jan 31;91(1):e0200424. doi: 10.1128/aem.02004-24. Epub 2024 Dec 23.
Antibiotic secretion plays a pivotal role in bacterial interference competition; yet, the impact of environmental hydration conditions on such competition is not well understood. Here, we investigate how hydration conditions affect interference competition among bacteria, studying the interactions between the antibiotic-producing FZB42 and two bacterial strains susceptible to its antibiotics: 85-10 and DC3000. Our results show that wet-dry cycles significantly modify the response of the susceptible bacteria to both the supernatant and cells of the antibiotic-producing bacteria, compared to constantly wet conditions. Notably shows increased protection against both the cells and supernatants of under wet-dry cycles, while cells become more susceptible under wet-dry cycles. In addition, we observed a reciprocal interaction between and , where inhibits under wet conditions. Our findings highlight the important role of hydration conditions in shaping bacterial interference competition, providing valuable insights into the microbial ecology of water-unsaturated surfaces, with implications for applications such as biological control of plant pathogens and mitigating antibiotic resistance.IMPORTANCEOur study reveals that hydration conditions, particularly wet-dry cycles, significantly influence antibiotic-mediated competition between bacterial species. We revealed that the effectiveness of antibiotics produced by against two susceptible bacterial species: and varies based on these hydration conditions. Unlike traditional laboratory environments, many real-world habitats, such as soil, plant surfaces, and even animal skin, undergo frequent wet-dry cycles. These conditions affect bacterial competition dynamics and outcomes, with wet-dry cycles providing increased protection for some bacteria while making others more susceptible. Our findings highlight the importance of considering environmental hydration when studying microbial interactions and developing biological control strategies. This research has important implications for improving agricultural practices and understanding natural microbial ecosystems.
抗生素分泌在细菌干扰竞争中起着关键作用；然而，环境水合条件对这种竞争的影响尚未得到充分理解。在这里，我们研究水合条件如何影响细菌之间的干扰竞争，研究产抗生素的FZB42与两种对其抗生素敏感的细菌菌株：85 - 10和DC3000之间的相互作用。我们的结果表明，与持续湿润条件相比，干湿循环显著改变了敏感细菌对产抗生素细菌的上清液和细胞的反应。值得注意的是，在干湿循环下，对产抗生素细菌的细胞和上清液都表现出增强的保护作用，而在干湿循环下细胞变得更易受影响。此外，我们观察到85 - 10和DC3000之间存在相互作用，其中85 - 10在湿润条件下抑制DC3000。我们的研究结果突出了水合条件在塑造细菌干扰竞争中的重要作用，为非饱和水表面的微生物生态学提供了有价值的见解，对植物病原体的生物防治和减轻抗生素耐药性等应用具有重要意义。重要性我们的研究表明，水合条件，特别是干湿循环，显著影响细菌物种之间由抗生素介导的竞争。我们发现FZB42产生的抗生素对两种敏感细菌物种：85 - 10和DC3000的有效性因这些水合条件而异。与传统实验室环境不同，许多现实世界的栖息地，如土壤、植物表面，甚至动物皮肤，都会经历频繁的干湿循环。这些条件影响细菌竞争动态和结果，干湿循环为一些细菌提供了增强的保护，而使其他细菌更易受影响。我们的研究结果强调了在研究微生物相互作用和制定生物防治策略时考虑环境水合的重要性。这项研究对改进农业实践和理解自然微生物生态系统具有重要意义。
