冷藏、室温及处理时间对犬新孢子虫血清免疫荧光抗体效价的影响。

Effect of refrigeration, room temperature, and processing time on serum immunofluorescent antibody titers for Sarcocystis neurona.

作者信息

Valderrama-Martinez Claudia, Packham Andrea, Zheng Shichen, Smith Woutrina, Plancarte Magdalena, Aleman Monica

机构信息

Departamento de Salud Animal, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional de Colombia, Bogotá, Colombia.

One Health Institute, School of Veterinary Medicine, University of California Davis, Davis, California, USA.

出版信息

J Vet Intern Med. 2025 Jan-Feb;39(1):e17282. doi: 10.1111/jvim.17282.

DOI:10.1111/jvim.17282

PMID:39715359

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11665959/

Abstract

BACKGROUND

Evaluating antibody titers for Sarcocystis neurona for the diagnosis of equine protozoal myeloencephalitis from serum samples is a common practice. However, ensuring timely and proper refrigeration is not always possible.

OBJECTIVES

To evaluate immunofluorescent antibody (IFA) titers for S. neurona from serum samples stored at room temperature and 4°C.

SAMPLES

Twenty-two serum samples.

METHODS

Prospective longitudinal study. Two serum aliquots of 1 mL each were stored at room temperature (20-23.3°C) and 4°C. The unrefrigerated aliquot was immediately tested for IFA titers. Both aliquots were retested on Days 5 and 10 after collection. A paired t test was used to compare IFA titers at different time points.

RESULTS

There was no significant difference between IFA titers from baseline with those stored at room temperature at Days 5 (P = .741, 95% CI [-56.83, 78.65]), 10 (P = .677, 95% CI [-50.01, 75.46]), and between 5 and 10 days (P = 0.949, 95% CI [-57.50, 61.14]). There was no significant difference from baseline with those stored at 4°C for Days 5 (P = .964, 95% CI [-81.81, 85.45]), 10 (P = 0.573, 95% CI [-109.4, 62.15]), and between 5 and 10 days (P = .5, 95% CI [-102.6, 51.67]). There was no statistical difference between samples stored at room temperature and 4°C (P = .688, CI [-55.51, 37.33]) on Days 5 and 10 (P = .104, CI [-80.8, 8.07]).

CONCLUSIONS AND CLINICAL IMPORTANCE

Immunofluorescent antibody test titers for S. neurona are stable for up to 10 days at room temperature and 4°C.

摘要

背景

通过检测血清样本中神经元肉孢子虫的抗体滴度来诊断马的原虫性脑脊髓炎是一种常见做法。然而，并非总能确保及时且恰当地冷藏样本。

目的

评估室温及4℃保存的血清样本中神经元肉孢子虫的免疫荧光抗体（IFA）滴度。

样本

22份血清样本。

方法

前瞻性纵向研究。将每份1 mL的两份血清分装分别保存在室温（20 - 23.3℃）和4℃。未冷藏的分装样本立即检测IFA滴度。两份分装样本在采集后第5天和第10天再次检测。采用配对t检验比较不同时间点的IFA滴度。

结果

室温保存样本在第5天（P = 0.741，95%可信区间[-56.83, 78.65]）、第10天（P = 0.677，95%可信区间[-50.01, 75.46]）以及第5天至第10天（P = 0.949，95%可信区间[-57.50, 61.14]）时，其IFA滴度与基线相比无显著差异。4℃保存样本在第5天（P = 0.964，95%可信区间[-81.81, 85.45]）、第10天（P = 0.573，95%可信区间[-109.4, 62.15]）以及第5天至第10天（P = 0.5，95%可信区间[-102.6, 51.67]）时，其IFA滴度与基线相比也无显著差异。在第5天和第10天，室温保存样本与4℃保存样本之间无统计学差异（P = 0.688，可信区间[-55.51, 37.33]）（P = 0.104，可信区间[-80.8, 8.07]）。