Chen Kai, Jiang Jun, Tian Yanlin, Guo Yu, He Tianyu, Xie Yuerou, Wu Kao, Zhu Fan, Jiang Fatang
National "111" Center for Cellular Regulation and Molecular Pharmaceutics, Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei University of Technology, Wuhan 430068, China; Hubei Key Laboratory of Industrial Microbiology, Hubei University of Technology, Wuhan 430068, China.
National "111" Center for Cellular Regulation and Molecular Pharmaceutics, Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei University of Technology, Wuhan 430068, China.
Int J Biol Macromol. 2025 Feb;291:139080. doi: 10.1016/j.ijbiomac.2024.139080. Epub 2024 Dec 21.
Biopolymer-based emulsion systems have been used for food preservation. In this study, mung bean protein (MBP) was added to konjac glucomannan (KGM)/curdlan-based camellia oil emulsion (KC-CO) to develop KCM-CO emulsion system. KCM-CO emulsions showed good compatibility and stability during storage. The confocal laser scanning microscopy, atomic force microscope, and infrared spectroscopy revealed that camellia oil was successfully emulsified by MBP, and the resulting droplets were evenly distributed in the polysaccharide network formed by KGM and curdlan micelles based on hydrogen bonds. The emulsions behaved as an elastic solid, and the KCM-CO emulsion films exhibited a compact microstructure, and the emulsification of MBP enhanced the compatibility, as KCM-CO had the smoothest surface. The addition of MBP significantly improved the elongation at break (EAB), water contact angle (WCA), dissolution, and gas permeability of the emulsion films. KCM-CO showed the largest EAB (37.6 %), strong hydrophobicity (WCA = 97.8°), and low water vapor and oxygen permeability. In the preservation experiments, KCM-CO coating significantly delayed the weight loss (by 41.2 %) and firmness decline (by 54.5 %), and maintained the appearance, total solids, total acids, and ascorbic acid content of cherry tomatoes, and inhibited the respiratory intensity by 44.2 %. This coating showed great potential for fruit and vegetable preservation.
基于生物聚合物的乳液体系已被用于食品保鲜。在本研究中,将绿豆蛋白(MBP)添加到基于魔芋葡甘露聚糖(KGM)/凝胶多糖的山茶油乳液(KC-CO)中,以开发KCM-CO乳液体系。KCM-CO乳液在储存期间表现出良好的相容性和稳定性。共聚焦激光扫描显微镜、原子力显微镜和红外光谱表明,山茶油被MBP成功乳化,所得液滴均匀分布在基于氢键由KGM和凝胶多糖胶束形成的多糖网络中。乳液表现为弹性固体,KCM-CO乳液膜呈现致密的微观结构,MBP的乳化增强了相容性,因为KCM-CO具有最光滑的表面。MBP的添加显著提高了乳液膜的断裂伸长率(EAB)、水接触角(WCA)、溶解性和气体渗透性。KCM-CO表现出最大的EAB(37.6%)、强疏水性(WCA = 97.8°)以及低水蒸气和氧气渗透性。在保鲜实验中,KCM-CO涂层显著延迟了樱桃番茄的失重(41.2%)和硬度下降(54.5%),保持了樱桃番茄的外观、总固形物、总酸和抗坏血酸含量,并抑制了呼吸强度44.2%。这种涂层在果蔬保鲜方面显示出巨大潜力。