Sahu Dileshwari, Khute Madhuri, Pillai Ajai Kumar
Govt. V.Y.T. Post Graduate Autonomous College Durg-491001 Chhattisgarh India
Government Nagarjuna Post Graduate College of Science Raipur-492010 Chhattisgarh India.
RSC Adv. 2024 Dec 23;14(54):40160-40172. doi: 10.1039/d4ra05313c. eCollection 2024 Dec 17.
The optical detection of arsenic (As) in human biological fluids and environmental water samples is presented using alpha-cyclodextrin-modified silver nanoparticles (α/CyD-AgNPs) at the trace level. This method is based on the measurement of a red shift of the LSPR band of α/CyD-AgNPs in the region of 200-800 nm. The color of α/CyD-AgNPs was changed from yellow to colorless by the addition of As(iii). The mechanism for the detection of As(iii) is based on the electrostatic interaction between the positively charged As(iii), which causes the red shift of the LSPR band from 410 nm to 580 nm. As(iii) ions specifically interact with α/CyD-AgNPs to form As(iii)-α/CyD-AgNPs and cause a remarkable decrease in the absorbance peak of AgNPs at 410 nm, which enables the determination of As(iii) with high selectivity and sensitivity. The interaction between α/CyD-AgNPs and As(iii) is theoretically explored by density functional theory (DFT) using LANL2DZ basis sets with the help of the Gaussian 09 (C.01) program. The developed colorimetric method provides a detection limit of 12.5 ppm with a detection range of 20-500 μg mL for As(iii) determination. The advantages of using α/CyD-AgNPs as a chemical sensor in colorimetry assays are that they are simple, low-cost and selective for the detection of As(iii) from human blood, serum, urine, and environmental river and tap water samples.
本文介绍了使用α-环糊精修饰的银纳米颗粒（α/CyD-AgNPs）对人体生物体液和环境水样中的痕量砷（As）进行光学检测的方法。该方法基于测量α/CyD-AgNPs在200-800nm区域的局域表面等离子体共振（LSPR）带的红移。加入As(iii)后，α/CyD-AgNPs的颜色从黄色变为无色。检测As(iii)的机制基于带正电荷的As(iii)之间的静电相互作用，这导致LSPR带从410nm红移至580nm。As(iii)离子与α/CyD-AgNPs特异性相互作用形成As(iii)-α/CyD-AgNPs，并导致AgNPs在410nm处的吸收峰显著降低，从而能够高选择性和高灵敏度地测定As(iii)。借助高斯09（C.01）程序，使用LANL2DZ基组通过密度泛函理论（DFT）从理论上探索了α/CyD-AgNPs与As(iii)之间的相互作用。所开发的比色法测定As(iii)的检测限为12.5ppm，检测范围为20-500μg/mL。在比色分析中使用α/CyD-AgNPs作为化学传感器的优点是它们简单、低成本且对从人体血液、血清、尿液以及环境河水和自来水样品中检测As(iii)具有选择性。
